Abstract |
We have previously demonstrated that two distinct transcripts are produced from ORF 50, the major transcriptional activating gene of herpesvirus saimiri. The products of these transcripts trans-activate the delayed-early ORF 6 promoter, though to different degrees. Deletion analysis demonstrated that the ORF 50 responsive elements are contained in a 132 bp fragment situated 127-259 bp from the transcription initiation site within the ORF 6 promoter. This fragment conferred ORF 50-responsiveness on an enhancerless simian virus 40. Gel retardation analysis further mapped the responsive elements to a 38 bp fragment.
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Authors | A Whitehouse, A J Stevenson, M Cooper, D M Meredith |
Journal | The Journal of general virology
(J Gen Virol)
Vol. 78 ( Pt 6)
Pg. 1411-5
(Jun 1997)
ISSN: 0022-1317 [Print] England |
PMID | 9191938
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Base Sequence
- Herpesvirus 2, Saimiriine
(genetics)
- Molecular Sequence Data
- Open Reading Frames
- Promoter Regions, Genetic
- Transcriptional Activation
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