Quantitation of beta-lapachone and 3-hydroxy-beta-lapachone in human plasma samples by reversed-phase high-performance liquid chromatography.

Abstract

beta-Lapachone is an o-naphthoquinone found to have in vitro cytotoxicity in cancer cells, type I human immunodeficiency virus, and fungi. Analytical methods for evaluating beta-lapachone in biological fluids using high-performance liquid chromatography (HPLC) have not been published. The reversed-phase HPLC method described in this report utilizes liquid extraction of a 0.5-ml plasma sample with average recoveries of 67+/-10.8% and 70+/-10.3% for beta-lapachone and 3-hydroxy-beta-lapachone, respectively. Sensitivity of the assay using ultraviolet (UV) detection at 256 nm is 15 ng ml(-1) from a 100 microl injection. Plasma standards for beta-lapachone and 3-hydroxy-beta-lapachone are linear with no significant difference in slope between the compounds. The retention times of 2.7 min for 3-hydroxy-beta-lapachone and 5.9 min for beta-lapachone result in a clean separation permitting use of the same assay procedure without modification for both compounds. This assay offers the advantage that either beta-lapachone or 3-hydroxy-beta-lapachone can serve as the internal standard, depending on which compound is being analyzed.

• Authors: V L Glen, P R Hutson, N J Kehrli, D A Boothman, G Wilding
• Journal: Journal of chromatography. B, Biomedical sciences and applications (J Chromatogr B Biomed Sci Appl) Vol. 692 Issue 1 Pg. 181-6 (Apr 25 1997) ISSN: 1387-2273 [Print] Netherlands
• PMID: 9187398 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 3-hydroxylapachone
• Anti-Infective Agents
• Antibiotics, Antineoplastic
• Naphthoquinones
• beta-lapachone

Topics

• Anti-Infective Agents (blood)
• Antibiotics, Antineoplastic (blood)
• Chromatography, High Pressure Liquid
• Humans
• Naphthoquinones (blood)