HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Isolation and characterization of a novel gene from human glioblastoma multiforme tumor tissue.

Abstract
Using the technique of DD-PCR (differential display-polymerase chain reaction) we isolated a novel gene (D2-2) that is overexpressed in glioblastoma multiforme tissue (GMT) as compared to normal brain tissue (NBT). D2-2 is also highly expressed in recurrent glioma, colon tumor metastatic to brain, breast tumors, prostate tumors and a prostate tumor cell line (LNCaP). Northern blot analysis showed that D2-2 is highly expressed in several tumor cell lines (MOLT lymphoblastic leukemia, SW480 colorectal adrenocarcinoma, A549 lung carcinoma, HL-60 promyelocytic leukemia, S3 HeLa cells, K-562 chronic myelogeneous leukemia and G361 melanoma) as compared to NBT. Additionally, D2-2 is very highly expressed in cell lines derived from glioblastomas, grade IV astrocytomas, normal human fetal astrocytes (NHFA) and glioma. D2-2 is moderately expressed in neuroblastoma, neuroectodermal and medulloblastoma tumor cell lines. D2-2 expression is localized to the frontal lobe, occipital lobe and the cerebellum in the normal brain. Normal tissues such as thyroid, stomach, adrenal cortex, small intestine and pancreas show high expression of D2-2. We also show that D2-2 is expressed 28-fold higher in fetal brain (20 weeks) than in adult brain. Sequence analysis of a 2.0-kb fragment for D2-2 shows no homology to known sequences in the data base.
AuthorsA Sehgal, C Keener, A L Boynton, R F Young, S S Vermeulen, K S Yonemura, E P Kohler, H C Aldape, C R Simrell, G P Murphy
JournalInternational journal of cancer (Int J Cancer) Vol. 71 Issue 4 Pg. 565-72 (May 16 1997) ISSN: 0020-7136 [Print] United States
PMID9178809 (Publication Type: Comparative Study, Journal Article)
Topics
  • Amino Acid Sequence
  • Astrocytes (metabolism)
  • Base Sequence
  • Brain Neoplasms (genetics, metabolism)
  • Genes
  • Glioblastoma (genetics, metabolism)
  • HL-60 Cells
  • HeLa Cells
  • Humans
  • Lymphoma, B-Cell (genetics, metabolism, pathology)
  • Meningeal Neoplasms (genetics, metabolism)
  • Meningioma (genetics, metabolism)
  • Molecular Sequence Data
  • Neoplasms (pathology)
  • Polymerase Chain Reaction
  • Subtraction Technique
  • Tumor Cells, Cultured

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: