Phenotypic and functional characterization of a new human macrophage cell line K1m demonstrating immunophagocytic activity and signalling through HLA class II.

Abstract

A human macrophage line, designated K1m, has been established from peripheral blood. K1m expresses a number of lineage-specific markers as well as a broad array of intercellular adhesion molecules. In particular, K1m expresses high levels of human leucocyte antigen (HLA) class I and class II. In response to ligation of HLA class II (HLA-DR), but not in response to ligation of HLA class 1, K1m forms tighter homotypic aggregates and develops a striking 'stellate' culture phenotype. K1m also expresses Fc receptors for immunoglobulin G (IgG) (CD64, CD32, and CD16) and can be shown to phagocytose polystyrene latex beads, as well as neuroblastoma cells in the presence of tumour-specific monoclonal antibody (mAb). The K1m cell line should therefore prove useful for studying both signalling through macrophage HLA class II and immunophagocytosis.

• Authors: D P Dialynas, M J Lee, L E Shao, P C Tan, J Yu
• Journal: Immunology (Immunology) Vol. 90 Issue 4 Pg. 470-6 (Apr 1997) ISSN: 0019-2805 [Print] England
• PMID: 9176097 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antigens, Surface
• HLA-DR Antigens

Topics

• Antibody-Dependent Cell Cytotoxicity
• Antigens, Surface (analysis)
• Cell Line (immunology)
• Flow Cytometry
• HLA-DR Antigens (immunology)
• Humans
• Immunophenotyping
• Macrophage Activation (immunology)
• Macrophages (immunology)
• Phagocytosis (immunology)
• Signal Transduction (immunology)