Determination of 4-hydroxyifosfamide in biological matrices by high-performance liquid chromatography.

Abstract

A high-performance liquid chromatographic method has been developed for the determination of 4-hydroxyifosfamide, a metabolite of ifosfamide, in plasma of cancer patients. The analyte is derivatized to 7-hydroxyquinoline, which can be detached fluorimetrically. The calibration graph is linear in the concentration range 0.05-25 microM, the limit of detection being 40 nM. Any inference from acrolein, another metabolite of ifosfamide, was ruled out. 4-Hydroxyifosfamide is very unstable in plasma and a stabilization procedure by adding citric acid has been developed. Thus treated, the samples were stable for 4 days. Analysis of a patient's plasma samples revealed that the 4-hydroxifosfamide concentration did not exceed 10 microM.

• Authors: G P Kaijser, P G Ter Riet, J de Kraker, A Bult, J H Beijnen, W J Underberg
• Journal: Journal of pharmaceutical and biomedical analysis (J Pharm Biomed Anal) Vol. 15 Issue 6 Pg. 773-81 (Mar 1997) ISSN: 0731-7085 [Print] England
• PMID: 9172103 (Publication Type: Journal Article)

Chemical References

• 4-hydroxyifosfamide
• Ifosfamide

Topics

• Cerebellar Neoplasms (blood)
• Child
• Chromatography, High Pressure Liquid
• Drug Stability
• Humans
• Ifosfamide (analogs & derivatives, blood)
• Medulloblastoma (blood)
• Reproducibility of Results
• Sensitivity and Specificity