HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Daidzein sulfoconjugates are potent inhibitors of sterol sulfatase (EC 3.1.6.2).

Abstract
Recent studies have associated high dietary isoflavone intake with low incidence of breast cancer. Since estrogenic steroids are important factors in the evolution of breast cancer, and in breast tumors they are derived mainly from the sterol sulfatase pathway, we have therefore investigated effects of the isoflavone daidzein and its sulfoconjugates, daidzein-4'-O-sulfate and daidzein-7,4'-di-O-sulfate, on sterol sulfatase acitivity using dehydroepiandrosterone sulfate as substrate. While daidzein does not affect sterol sulfatase, its sulfoconjugates are potent inhibitors of this enzyme. Kinetic analyses reveal that daidzein-4'-O-sulfate and daidzein-7,4'-di-O-sulfate inhibit sterol sulfatase competitively with respect to the steroid substrate and with Ki values of 5.9 and 1 microM, respectively. Daidzein sulfo-conjugates also inhibit hydroxysteroid and phenol sulfotransferases but at much higher concentrations. These results provide a biochemical basis for the putative chemopreventive role of dietary isoflavones against breast cancer.
AuthorsC K Wong, W M Keung
JournalBiochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 233 Issue 3 Pg. 579-83 (Apr 28 1997) ISSN: 0006-291X [Print] UNITED STATES
PMID9168894 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Enzyme Inhibitors
  • Estrogen Antagonists
  • Isoflavones
  • Sulfuric Acid Esters
  • daidzein-4'-O-sulfate
  • daidzein-7,4'-di-O-sulfate
  • daidzein
  • Sulfotransferases
  • alcohol sulfotransferase
  • Arylsulfatases
  • Steryl-Sulfatase
Topics
  • Animals
  • Arylsulfatases (antagonists & inhibitors)
  • Breast Neoplasms (prevention & control)
  • Cricetinae
  • Cytosol (enzymology)
  • Diet
  • Enzyme Inhibitors (pharmacology)
  • Estrogen Antagonists (pharmacology)
  • Female
  • Humans
  • In Vitro Techniques
  • Isoflavones (pharmacology)
  • Kinetics
  • Liver (enzymology)
  • Mesocricetus
  • Steryl-Sulfatase
  • Sulfotransferases (antagonists & inhibitors)
  • Sulfuric Acid Esters (pharmacology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: