A xanthine oxidase inhibitor 1'-acetoxychavicol acetate inhibits azoxymethane-induced colonic aberrant crypt foci in rats.

Abstract

The modifying effect of dietary administration of a xanthine oxidase inhibitor 1'-acetoxychavicol acetate (ACA) present in an edible plant Languas galanga in Thailand on the development of azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) was investigated in rats. Male F344 rats were given s.c. injections of AOM (15 mg/kg body wt) once a week for 3 weeks to induce colonic ACF. They were fed the diets containing 100 or 200 ppm ACA for 5 weeks, starting 1 week before the first dosing of AOM. At the termination of the study (week 5), AOM induced 118 +/- 28 ACF/colon. Dietary administration of ACA caused significant reduction in the frequency of ACF (41% inhibition by 100 ppm ACA feeding and 37% inhibition by 200 ppm ACA feeding, P<0.01). Such inhibition might be associated with suppression of the proliferation biomarkers' expression such as ornithine decarboxylase activity in the colonic mucosa, number of silver-stained nucleolar organizer regions' protein in the colonic mucosal cell nuclei and blood polyamine content. These results indicate that ACA could inhibit the development of AOM-induced ACF through its suppression of cell proliferation in the colonic mucosa and ACA might be a possible chemopreventive agent against colon tumourigenesis.

• Authors: T Tanaka, H Makita, T Kawamori, K Kawabata, H Mori, A Murakami, K Satoh, A Hara, H Ohigashi, K Koshimizu
• Journal: Carcinogenesis (Carcinogenesis) Vol. 18 Issue 5 Pg. 1113-8 (May 1997) ISSN: 0143-3334 [Print] England
• PMID: 9163704 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Benzyl Alcohols
• Enzyme Inhibitors
• Polyamines
• Terpenes
• Xanthine Oxidase
• Ornithine Decarboxylase
• Azoxymethane
• 1'-acetoxychavicol acetate

Topics

• Animals
• Antineoplastic Agents (pharmacology)
• Azoxymethane
• Benzyl Alcohols
• Body Weight
• Cell Division (drug effects)
• Colon (drug effects, pathology)
• Colonic Diseases (chemically induced)
• Enzyme Inhibitors (pharmacology)
• Intestinal Mucosa (enzymology)
• Liver (anatomy & histology)
• Male
• Organ Size
• Ornithine Decarboxylase (metabolism)
• Polyamines (blood)
• Precancerous Conditions (chemically induced)
• Rats
• Rats, Inbred F344
• Terpenes (pharmacology)
• Xanthine Oxidase (antagonists & inhibitors)