Many chemicals have recently been discovered to have estrogenic activity, including the
surfactant intermediate
nonylphenol (NP). It has been well documented that
estrogen is a facilitator of human
breast cancer development under certain conditions, and environmental
estrogens such as NP are currently under intense investigation. Using the expression of pS2 (a
trefoil peptide expressed in
breast cancer cells), MUC1 (a member of the
mucin family) and ER (the human
estrogen receptor) genes as
estrogen-responsive reporter genes, the effects of
estradiol and NP on human
breast cancer cells-MCF-7 were studied. In the time course study, the
mRNA expressions were detected after
NP (10 microM) or
estradiol (E2, 0.1 microM) treatments using the RT-PCR technique. The results indicated: (1) NP and E2 induced pS2
mRNA expression after a 2-h exposure and (2) NP induction produced the highest level of MUC1
mRNA after 2 h, which was reduced to only 42% of control at 48 h. E2 treatment resulted in a gradual increase in MUC1 expression over the course of the exposure. The highest level of MUC1
mRNA was at 48 h. This indicates that NP may stimulate MUC1 expression by a different mechanism than E2. (3) NP affected ER expression in the same manner as MUC1. In contrast, E2 stimulated ER expression in a similar manner as pS2; the highest level was at 2 h and expression remained elevated through the 48-h point. NP is an estrogenic compound that alters pS2, MUC1 and ER gene expression in MCF-7 cells. NP may affect MUC1 expression through a different mechanism than E2. The link between aberrant MUC1, PS2 and ER expression and the development of
breast cancer also needs to be elucidated through further investigation.