| Abstract | We have studied the site of fibrinogen localization in normal platelets and in the platelets of a patient with congenital afibrinogenemia (CA). The methods employed were: direct immunofluorescence technique (DIT) and indirect immunofluorescence technique (IIT). By means of the DIT normal platelets were shown to have a clear peripheral staining. Such staining disappeared after treatment with proteolytic enzymes and after specific blocking experiments. Such peripheral staining of platelets was absent in congenital afibrinogenemia even after fibrinogen infusion. By means of the IIT platelets were shown to have a considerable amount of fibrinogen. Such protein was demonstrated to represent an important part of platelet surface, since intact platelets were able to absorb completely a specific antifibrinogen antiserum. |
| Authors | F Fabris, C Betterle, A Casonato, A Girolami |
| Journal | Folia haematologica (Leipzig, Germany : 1928)
(Folia Haematol Int Mag Klin Morphol Blutforsch)
Vol. 106
Issue 2
Pg. 260-8
( 1979)
ISSN: 0323-4347 GERMANY, EAST |
| PMID | 91563
(Publication Type: Journal Article)
|
| Chemical References |
|
| Topics |
- Afibrinogenemia
(blood)
- Blood Platelets
(analysis, drug effects)
- Fibrinogen
(isolation & purification)
- Fluorescent Antibody Technique
- Humans
- Trypsin
(pharmacology)
|
