Abstract |
The photodynamic effect of Victoria blue BO ( VB-BO) and photoirradiation on peripheral blood mononuclear cells was studied. The cells were preincubated with VB-BO followed by photoirradiation and overnight culture. The highest percentage of dead cells ( propidium iodide assay in flow cytometry) was seen in the monocyte population. The lymphocytes showed a lower sensitivity to VB-BO photodynamic action than the monocytes (12% vs 80% of PI-positive cells). The effect of VB-BO and phototreatment on lymphocyte function was studied using a mitogen-induced proliferation assay. A decrease of mitogen response was observed. The VB-BO and photoirradiation were also used on leukemic cells. The leukemic cells from acute myeloid leukemia and B precursors leukemia were sensitive to VB-BO photodynamic action. The high VB-BO sensitivity of monocytes and leukemic cells (myeloid and lymphoid B derived) suggests possible application of VB-BO for selective depletion of monocytes or sensitive leukemic cells.
|
Authors | M Fiedorowicz, A Pituch-Noworolska, M Zembala |
Journal | Photochemistry and photobiology
(Photochem Photobiol)
Vol. 65
Issue 5
Pg. 855-61
(May 1997)
ISSN: 0031-8655 [Print] United States |
PMID | 9155257
(Publication Type: Journal Article)
|
Chemical References |
- Antineoplastic Agents
- Antiviral Agents
- Fluorescent Dyes
- Photosensitizing Agents
- Pyrimidinones
- Quaternary Ammonium Compounds
- Victoria blue BO
- merocyanine dye
|
Topics |
- Antineoplastic Agents
(pharmacology)
- Antiviral Agents
(pharmacology)
- Bone Marrow Cells
- Cell Survival
(drug effects, radiation effects)
- Fluorescent Dyes
- Humans
- In Vitro Techniques
- Leukemia
(pathology)
- Leukocytes, Mononuclear
(drug effects, radiation effects)
- Monocytes
(drug effects, radiation effects)
- Photochemistry
- Photosensitizing Agents
(pharmacology)
- Pyrimidinones
(pharmacology)
- Quaternary Ammonium Compounds
(pharmacology)
- Radiation Tolerance
(drug effects)
|