Renal cell carcinoma and normal kidney protein expression.

Abstract

Renal cell carcinoma (RCC), a human kidney cancer from the proximal tubular epithelium, accounts for about 3% of adult malignancies. Molecular and cytogenetic analysis have highlighted deletions, translocations, or loss of heterozygosity in the 3p21-p26, a putative RCC locus, as well as in 6q, 8p, 9pq, and 14pq. Studies on phenotypic expression of human kidney tissue and on post-translational modifications in RCC have not yet provided a marker for early renal cell carcinoma diagnosis. Current diagnostic methods do not help to detect the tumor before advanced stages. We therefore used two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) to study normal and tumor kidney tissues in ten patients suffering from RCC. A human kidney protein map in the SWISS-2DPAGE database accessible through the ExPASy WWW Molecular Biology Server was established. Of 2789 separated polypeptides, 43 were identified by gel comparison, amino acid analysis, N-terminal sequencing, and/or immunodetection. The comparison between normal and tumor kidney tissues showed four polypeptides to be absent in RCC. One of them was identified as ubiquinol cytochrome c reductase (UQCR), whose locus has elsewhere been tentatively assigned to chromosome 19p12 or chromosome 22. A second polypeptide was identified as mitochondrial NADH-ubiquinone oxido-reductase complex I whose locus is located on chromosome 18p11.2 and chromosome 19q13.3. These result suggest that the lack of UQCR and of mitochondrial NADH-ubiquinone oxidoreductase complex I expression in RCC may be caused by unknown deletions, or by changes in gene transcription or translation. It might indicate that mitochondrial disfunction plays a major role in RCC genesis or evolution.

• Authors: C Sarto, A Marocchi, J C Sanchez, D Giannone, S Frutiger, O Golaz, M R Wilkins, G Doro, F Cappellano, G Hughes, D F Hochstrasser, P Mocarelli
• Journal: Electrophoresis (Electrophoresis) 1997 Mar-Apr Vol. 18 Issue 3-4 Pg. 599-604 ISSN: 0173-0835 [Print] Germany
• PMID: 9150947 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Neoplasm Proteins

Topics

• Amino Acid Sequence
• Carcinoma, Renal Cell (chemistry, pathology)
• Electrophoresis, Gel, Two-Dimensional
• Humans
• Kidney Neoplasms (chemistry, pathology)
• Kidney Tubules, Proximal (chemistry, pathology)
• Molecular Sequence Data
• Neoplasm Proteins (analysis)
• Peptide Mapping