Volatile substances in blood serum: profile analysis and quantitative determination.

Abstract

Alcohols, ketones and aldehydes have been identified in the profiles of volatile substances in blood serum. A 5-ml sample is required in order to obtain a complete gas chromatographic profile, selective profiles of alcohols or individual ketones by computer mass fragmentography, and to permit mass spectrometric identification of the compounds. The quantitative determination of total 4-heptanone using an extraction and gas chromatographic-mass fragmentographic procedure demonstrated concentrations of 10-50 nmole/l in normal serum, whereas in the serum of patients with chronic renal insufficiency the concentrations were 10- to 40-fold higher. Ethanol and total acetone were quantitatively measured by direct injection of serum. For 10 patients, concentrations between 25 and 85 mumole/l of acetone and 10-170 mumole/l of ethanol were determined.

• Authors: H M Liebich, J Wöll
• Journal: Journal of chromatography (J Chromatogr) Vol. 142 Pg. 505-16 (Nov 11 1977) Netherlands
• PMID: 914932 (Publication Type: Journal Article)

Chemical References

• Aldehydes
• Ketones
• Ethanol

Topics

• Aldehydes (blood)
• Computers
• Ethanol (blood)
• Gas Chromatography-Mass Spectrometry
• Humans
• Ketones (blood)
• Volatilization