Developmentally regulated expression of APG-1, a member of heat shock protein 110 family in murine male germ cells.

Abstract

Apg-1 encodes a heat shock protein belonging to the heat shock protein 110 family, and is inducible by a 32 degrees C to 39 degrees C heat shock. Northern blot analysis of the testis from immature and adult mice, and of the purified germ cells revealed the quantitative change of the apg-1 transcripts during germ cell development. By in situ hybridization histochemistry the expressions of the apg-1 transcripts were detected in germ cells at specific stages of development including spermatocytes and spermatids. Although heat-induction of the apg-1 transcripts was observed in W/Wv mutant testis lacking germ cells, it was not detected in wild-type testis nor in the purified germ cells. Thus, the apg-1 expression is not heat-regulated but developmentally regulated in germ cells, suggesting that APG-1 plays a role in normal development of germ cells.

• Authors: Y Kaneko, T Kimura, H Nishiyama, Y Noda, J Fujita
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 233 Issue 1 Pg. 113-6 (Apr 07 1997) ISSN: 0006-291X [Print] United States
• PMID: 9144406 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• HSP110 Heat-Shock Proteins
• HSP70 Heat-Shock Proteins
• Heat-Shock Proteins
• Osp94 protein, mouse
• RNA, Messenger

Topics

• Animals
• Gene Expression Regulation, Developmental
• HSP110 Heat-Shock Proteins
• HSP70 Heat-Shock Proteins (genetics)
• Heat-Shock Proteins (genetics)
• Hot Temperature
• In Situ Hybridization
• Male
• Mice
• Mice, Mutant Strains
• RNA, Messenger (genetics)
• Spermatogenesis (genetics)
• Spermatozoa (metabolism)
• Testis (cytology, metabolism)