Abstract |
The estrogen receptor (ER) gene is located on chromosome 6. The aim of our study was to investigate whether numerical chromosomal aberrations were reflected in estrogen/ progesterone receptor (PgR) status and staining pattern. Fine-needle aspirates from 51 breast carcinomas were investigated immunocytochemically for ER/PgR and by in situ hybridization technique using digoxigenin-labeled alpha-satellite probe for chromosome 6. Cases with > or = 70% two-signal nuclei were regarded as disome; the remaining tumors showed aneusomy with a variable number of signals. Aneusomy was found in 32 tumors (63%), whereas 19 (37%) had a normal number of chromosome 6. Chromosomal gain occurred in all aneusome cases except one. ER- and/or PgR-positive tumors had an equal distribution of disomy and aneusomy. Variable ER staining pattern or ER and/or PgR negativity was associated with numerical aberrations in chromosome 6 in 76% of the tumors. Cancers with uniform ER staining pattern all had normal chromosome number.
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Authors | T Sauer, K Beraki, P W Jebsen, E Ormerod, O Naess |
Journal | Diagnostic cytopathology
(Diagn Cytopathol)
Vol. 16
Issue 5
Pg. 420-4
(May 1997)
ISSN: 8755-1039 [Print] United States |
PMID | 9143843
(Publication Type: Journal Article)
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Chemical References |
- Coloring Agents
- DNA Probes
- Receptors, Estrogen
- Receptors, Progesterone
- Digoxigenin
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Topics |
- Biopsy, Needle
- Breast Neoplasms
(chemistry, genetics)
- Cell Nucleus
(chemistry)
- Chromosome Aberrations
- Chromosomes, Human, Pair 6
- Coloring Agents
- DNA Probes
- Digoxigenin
- Female
- Humans
- In Situ Hybridization
- Receptors, Estrogen
(analysis)
- Receptors, Progesterone
(analysis)
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