Inhibition by protein kinase C inhibitor of expression of leukocyte function-associated antigen-1 molecules in rat hepatoma AH66F cells.

Abstract

To examine the mechanism of inhibition by protein kinase C (PKC) inhibitors of the adhesion of highly malignant hepatoma AH66F cells to the mesentery-derived mesothelial cell (M-cell) layer through leukocyte function-associated antigen-1 (LFA-1)/intercellular adhesion molecule-1, the effects of a PKC inhibitor, NA-382, on the expression of LFA-1 molecules in AH66F cells were examined and compared with those in thymocytes from normal rats. NA-382 inhibited the adhesion of AH66F cells to the M-cell layer and the expression of LFA-1 on the membrane of the hepatoma cells after treatment for more than 24 h. It was confirmed that AH66F cells express similar mRNAs for LFA-1 subunits to those of thymocytes, and their levels were also decreased after treatment with NA-382. On the other hand, the LFA-1 mediated adhesion and the expression of both protein and mRNA for LFA-1 subunits in thymocytes were not changed by the PKC inhibitor. These results suggest that the expression of LFA-1 molecules in AH66F cells may be regulated by PKC via quite different mechanisms from those in normal lymphocytes.

• Authors: M Nomura, N Sugiura, S Moritani, K Miyamoto
• Journal: Japanese journal of cancer research : Gann (Jpn J Cancer Res) Vol. 88 Issue 3 Pg. 267-72 (Mar 1997) ISSN: 0910-5050 [Print] Japan
• PMID: 9140111 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Alkaloids
• DNA Primers
• Enzyme Inhibitors
• Lymphocyte Function-Associated Antigen-1
• Macromolecular Substances
• N-ethoxycarbonyl-7-oxostaurosporine
• Protein Kinase C
• Staurosporine

Topics

• Alkaloids (pharmacology)
• Animals
• Cell Adhesion (drug effects)
• DNA Primers
• Enzyme Inhibitors (pharmacology)
• Flow Cytometry
• Liver Neoplasms, Experimental (pathology, physiopathology)
• Lymphocyte Function-Associated Antigen-1 (biosynthesis)
• Macromolecular Substances
• Mesentery (cytology, drug effects, physiology)
• Polymerase Chain Reaction
• Protein Kinase C (antagonists & inhibitors)
• Rats
• Staurosporine (analogs & derivatives)
• T-Lymphocytes (metabolism)
• Tumor Cells, Cultured