Analyses of HLA-DQ alleles in recurrent spontaneous abortion (RSA) couples.

Abstract

PROBLEM:

The influence of HLA sharing on pregnancy outcome is controversial. In renal transplantation, HLA-DQB1 donor-recipient mismatches have been shown beneficial for long-term transplant success. Since pregnancy is defined as Nature's allograft, we investigated the relevance of HLA-DQ mismatching in normal reproducing couples compared to couples experiencing RSA.

METHOD:

Unexplained RSA couples referred to our laboratory for immunological testing were classified by immunological findings and obstetrical history. Primary RSA couples shared > or = 2 HLA-A, B, or DR antigens, had no cytotoxic anti-paternal antibodies, and no gestation beyond 20 weeks. Secondary RSA couples had cytotoxic anti-paternal antibodies and RSA after a live birth. HLA-DQA1 and DQB1 alleles were identified by PCR-SSP.

RESULTS:

No differences in DQA1 and DQB1 mismatch were observed among RSA patients and controls. DQA1-DQB1 haplotype mismatches were not different among the three groups of couples.

CONCLUSIONS:

In contrast to renal transplant, HLA-DQ incompatibility did not differ among RSA couples compared with successful reproducing couples.

• Authors: D R Wagenknecht, K M Green, J A McIntyre
• Journal: American journal of reproductive immunology (New York, N.Y. : 1989) (Am J Reprod Immunol) Vol. 37 Issue 1 Pg. 1-6 (Jan 1997) ISSN: 1046-7408 [Print] Denmark
• PMID: 9138442 (Publication Type: Journal Article)

Chemical References

• HLA-DQ Antigens
• HLA-DQ alpha-Chains
• HLA-DQA1 antigen

Topics

• Abortion, Habitual (genetics, immunology)
• Alleles
• Female
• Gene Frequency
• HLA-DQ Antigens (analysis)
• HLA-DQ alpha-Chains
• Haplotypes
• Histocompatibility Testing
• Humans
• Male
• Pregnancy