Abstract |
Syncytium formation is one of major cytopathic effects of human immunodeficiency virus ( HIV) infection, and requires the interaction of CD4 molecules on uninfected cells with HIV envelope glycoprotein gp120 expressed on HIV-infected cells. Recent evidence suggests chemokine receptors function as fusion cofactors. We have recently found that fusion regulatory protein (FRP)-1/ CD98 is involved in syncytium formation of HIV gp160-expressing U2ME-7 cells and TALL-1 cells persistently infected with HIV. However, resting lymphocytes were found to express no FRP-1 molecule. In this study, we demonstrated that recombinant gp120 (rpg120) has the ability to induce expression of FRP-1 on peripheral blood mononuclear cells (PBMC). Three-color flow cytometric analysis showed that rgp120-induced FRP-1 was expressed selectively on CD4+ T cells in a dose-dependent manner. FRP-1 expression level was maximum 3 days after addition of rgp120. Anti-CD4 and anti-gp120 antibodies blocked rgp120-induced FRP-1 expression. Co-cultivation of PBMC with HIV-1 gp160-expressing HeLa cells also resulted in the increased expression of FRP-1 on T cells. These results suggest that FRP-1 molecules are induced on CD4+ T cells via CD4-gp120 interaction and may play an important role in regulation of HIV-induced syncytium formation.
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Authors | S Suga, M Tsurudome, M Ito, S Ohgimoto, N Tabata, M Nishio, M Kawano, H Komada, M Ito, M Sakurai, Y Ito |
Journal | Medical microbiology and immunology
(Med Microbiol Immunol)
Vol. 185
Issue 4
Pg. 237-43
(Mar 1997)
ISSN: 0300-8584 [Print] Germany |
PMID | 9138296
(Publication Type: Journal Article)
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Chemical References |
- Antigens, CD
- Carrier Proteins
- Fusion Regulatory Protein-1
- HIV Envelope Protein gp120
- HIV Envelope Protein gp160
- Interleukin-2
- Concanavalin A
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Topics |
- Antigens, CD
(biosynthesis)
- CD4-Positive T-Lymphocytes
(drug effects, immunology, pathology)
- Carrier Proteins
(biosynthesis)
- Cell Fusion
- Coculture Techniques
- Concanavalin A
(pharmacology)
- Cytopathogenic Effect, Viral
- Flow Cytometry
- Fusion Regulatory Protein-1
- Giant Cells
- HIV Envelope Protein gp120
(immunology)
- HIV Envelope Protein gp160
(biosynthesis)
- HIV-1
(immunology)
- HeLa Cells
- Humans
- Interleukin-2
(pharmacology)
- Models, Immunological
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