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Influence of L-fucose attached alpha 1-->6 to the asparagine-linked N-acetylglucosamine on the hydrolysis of the N-glycosidic linkage by human glycosylasparaginase.

Abstract
The sequence of hydrolytic reactions in the catabolism of the N-glycosidic oligosaccharide-to-protein region containing 6-linked fucose on the asparagine-linked N-acetylglucosamine may vary from species to species. When alpha-L-fucopyranosyl-(1-->6)-2-acetamido-1-N-(beta-L-aspartyl)-2-deoxy- beta -D-glucopyranosylamine (Fuc-GlcNAc-Asn) was incubated with recombinant human glycosylasparaginase, no hydrolysis of the N-glycosidic bond was detected. After removal of the alpha 1-->6-linked fucose from the compound by alpha-fucosidase, the residual GlcNAc-Asn was rapidly hydrolyzed by glycosylasparaginase. Enzymologically this demonstrates for the first time that the catabolism of Fuc-GlcNAc-Asn in humans occurs via consecutive action of alpha-fucosidase and glycosylasparaginase. The hydrolysis rate of GlcNAc-Asn by glycosylasparaginase remained unaffected in the presence of Fuc-GlcNAc-Asn or several different monosaccharides including fucose. This indicates that any fucose attached alpha 1-->6 to the asparagine-linked N-acetylglucosamine residue prevents the access of the L-asparagine residue of Fuc-GlcNAc-Asn into the deep, funnel-shaped active site of human glycosylasparaginase. These findings explain the accumulation of fucosylated and normal catabolism of nonfucosylated glycoasparagines in fucosidosis.
AuthorsT Noronkoski, I Mononen
JournalGlycobiology (Glycobiology) Vol. 7 Issue 2 Pg. 217-20 (Mar 1997) ISSN: 0959-6658 [Print] England
PMID9134428 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Glycosides
  • Recombinant Proteins
  • Fucose
  • alpha-L-Fucosidase
  • Aspartylglucosylaminase
  • Acetylglucosamine
Topics
  • Acetylglucosamine (metabolism)
  • Aspartylglucosylaminase (genetics, metabolism)
  • Fucose (metabolism)
  • Fucosidosis (etiology)
  • Glycosides (metabolism)
  • Humans
  • Recombinant Proteins (metabolism)
  • Species Specificity
  • Substrate Specificity
  • alpha-L-Fucosidase (metabolism)

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