Immunohistochemically detected expression of motility-related protein-1 (MRP-1/CD9) in lung adenocarcinoma and its relation to prognosis.

Abstract

Motility-related protein-1 (MRP-1)/CD9 is a trans-membrane glycoprotein closely associated with suppression of cell motility and reduced metastatic potential of some tumor cells. We currently report that, according to the RT-PCR method for MRP-1/CD9 gene expression, patients with low expression of MRP-1/CD9 in non-small-cell lung cancer, especially the adenocarcinoma type, showed short overall survival. Then, to determine accurately the prognostic value of MRP-1/CD9 product levels in lung-adenocarcinoma cells, we immunohistochemically investigated its expression in 132 lung-adenocarcinoma patients undergoing potentially curative surgery. Of these patients, 44 (33%) showed reduced expression of MRP-1/CD9 in cancer cells, and an inverse association was observed between its expression and factors associated with tumor progression, such as nodal involvement (p = 0.029) or stage (p = 0.028). Patients with reduced expression of MRP-1/CD9 showed a significantly worse prognosis in overall survival (p = 0.005) and disease-free survival (DFS; p < 0.0001) than those with stronger expression; and even among patients with stage-I disease, similar results were obtained (overall survival, p = 0.038; DFS, p = 0.012). In a multivariate analysis, immunohistochemical MRP-1/CD9-expression level was an independent prognostic factor for DFS (p = 0.021), but not for overall survival (p = 0.572). Thus, immunohistochemical MRP-1/CD9-expression level solely in lung-adenocarcinoma cells within the tumor tissue appears to be a prognostic factor for DFS, and may be useful for detecting a high-risk sub-group of recurrence during the post-operative clinical course of the disease.

• Authors: M Higashiyama, O Doi, K Kodama, H Yokouchi, M Adachi, C L Huang, T Taki, T Kasugai, S Ishiguro, S Nakamori, M Miyake
• Journal: International journal of cancer (Int J Cancer) Vol. 74 Issue 2 Pg. 205-11 (Apr 22 1997) ISSN: 0020-7136 [Print] United States
• PMID: 9133457 (Publication Type: Journal Article)

Chemical References

• Antigens, CD
• CD9 protein, human
• Membrane Glycoproteins
• Neoplasm Proteins
• Tetraspanin 29

Topics

• Adult
• Aged
• Aged, 80 and over
• Antigens, CD (metabolism)
• Carcinoma, Non-Small-Cell Lung (metabolism, mortality, pathology)
• Female
• Humans
• Immunohistochemistry
• Lung Neoplasms (metabolism, mortality, pathology)
• Lymphatic Metastasis
• Male
• Membrane Glycoproteins (metabolism)
• Middle Aged
• Neoplasm Proteins (metabolism)
• Prognosis
• Survival Rate
• Tetraspanin 29