Cloning and mapping of human Rab7 and Rab9 cDNA sequences and identification of a Rab9 pseudogene.

Abstract	Rab GTPases reside in specific intracellular compartments and are key regulators of vesicular transport. To facilitate studies of the mechanism of lysosomal integral membrane protein (LAMP-1) transport, cDNAs for human Rab7 and Rab9 were isolated, and their nucleotide sequences were determined. During isolation and characterization of these cDNAs a Rab9 pseudogene was identified. The sequences are highly homologous to other mammalian Rab proteins and also share homology with proteins of the Rab GTPase family. Rab7 and the Rab9 pseudogene were mapped to chromosomes 3 and 5, respectively, by amplification of their sequences from human monochromosomal somatic cell hybrids. In addition, preliminary studies using antisense expression indicate that down-regulation of either Rab7 or Rab9 proteins induces severe cell vacuolation that resembles the phenotype seen in fibroblasts from patients with Chediak-Higashi syndrome.
Authors	J P Davies, P D Cotter, Y A Ioannou
Journal	Genomics (Genomics) Vol. 41 Issue 1 Pg. 131-4 (Apr 01 1997) ISSN: 0888-7543 [Print] United States
PMID	9126495 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References	DNA Primers DNA, Complementary Oligonucleotides, Antisense rab7 GTP-Binding Proteins rab7 GTP-binding proteins, human GTP Phosphohydrolases GTP-Binding Proteins RAB9A protein, human rab GTP-Binding Proteins
Topics	Animals Base Sequence Chediak-Higashi Syndrome (etiology) Chromosome Mapping Cloning, Molecular DNA Primers (genetics) DNA, Complementary (genetics) Dogs GTP Phosphohydrolases (antagonists & inhibitors, genetics) GTP-Binding Proteins (antagonists & inhibitors, genetics) HeLa Cells Humans Molecular Sequence Data Oligonucleotides, Antisense (pharmacology) Pseudogenes Vacuoles (drug effects) rab GTP-Binding Proteins rab7 GTP-Binding Proteins

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!