Effects of dichlobenil on ultrastructural morphology and cell replication in the mouse olfactory mucosa.

Abstract

The herbicide dichlobenil (2,6-dichlorobenzonitrile) is a tissue-specific inducing necrosis in the olfactory mucosa. Transmission electron microscopy showed vacuolations (1 hr) and necrosis (4 hr) in the Bowman's glands following intraperitoneal injection of dichlobenil (25 mg/kg) in C57B1 mice, whereas no lesions were noted in the olfactory neuroepithelium at these timepoints. Twenty-four hr following injection (25 mg/kg), most of the propria and the olfactory epithelium were severely damaged; the basal lamina, nerves, and blood vessels in the lamina propria, however, remained intact. Following a lower dose (12 mg/kg), the lesions were not as pronounced. The effects of dichlobenil (6, 12, and 25 mg/kg) on cell replication in the olfactory mucosa, as determined by incorporation of 3H-thymidine 3 days later, were more pronounced in the lamina propria than in the neuroepithelium and occurred at a lower dose in the lamina propria than in the neuroepithelium. Together these studies support the previous proposal that dichlobenil induces a primary damage in the Bowman's glands.

• Authors: M Mancuso, A Giovanetti, E B Brittebo
• Journal: Toxicologic pathology (Toxicol Pathol) 1997 Mar-Apr Vol. 25 Issue 2 Pg. 186-94 ISSN: 0192-6233 [Print] United States
• PMID: 9125777 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Benzamides
• Herbicides
• Nitriles
• Pharmaceutical Vehicles
• dichlobanil

Topics

• Animals
• Benzamides (toxicity)
• Cell Division (drug effects)
• Female
• Follow-Up Studies
• Herbicides (toxicity)
• Mice
• Mice, Inbred C57BL
• Necrosis
• Nitriles
• Olfactory Mucosa (drug effects, pathology, ultrastructure)
• Pharmaceutical Vehicles
• Vacuoles (drug effects, pathology, ultrastructure)