Expression of the Yersinia pestis capsular antigen (F1 antigen) on the surface of an aroA mutant of Salmonella typhimurium induces high levels of protection against plague.

Abstract	The caf operon from Yersinia pestis encoding the structural subunit (caf1), the molecular chaperone (caf1M), the outer membrane anchor (caf1A), and the regulatory protein (caf1R) was cloned into Salmonella typhimurium SL3261 aroA. The recombinant Salmonella organisms were encapsulated when cultured at 37 degrees C but not when cultured at 28 degrees C. Oral inoculation of mice with the recombinant Salmonella induced predominantly an immunoglobulin G2a response to F1 antigen, and isolated T cells showed a recall response to soluble or Salmonella-associated F1 antigen. Mice immunized with S. typhimurium SL3261 aroA expressing F1 antigen intracellularly developed lower antibody responses to F1 antigen and showed a T-cell recall response only to Salmonella-associated F1 antigen. Mice immunized orally with two doses of the recombinant Salmonella which expressed F1 antigen on the surface were protected against 10(7) 50% lethal doses (LD50) of virulent Y. pestis given by the subcutaneous route of challenge, whereas mice immunized with the recombinant Salmonella expressing F1 antigen intracellularly were only partially protected against 10(5) LD50 of Y. pestis.
Authors	R W Titball, A M Howells, P C Oyston, E D Williamson
Journal	Infection and immunity (Infect Immun) Vol. 65 Issue 5 Pg. 1926-30 (May 1997) ISSN: 0019-9567 [Print] United States
PMID	9125581 (Publication Type: Journal Article)
Chemical References	Bacterial Proteins Immunoglobulin G Vaccines, Synthetic caf1 protein, Yersinia pestis Transferases Alkyl and Aryl Transferases 3-Phosphoshikimate 1-Carboxyvinyltransferase
Topics	3-Phosphoshikimate 1-Carboxyvinyltransferase Administration, Oral Alkyl and Aryl Transferases Animals Bacterial Proteins (genetics, immunology) Blotting, Western Colony Count, Microbial Electrophoresis, Polyacrylamide Gel Female Fluorescent Antibody Technique, Indirect Gene Expression Regulation, Bacterial Immunoglobulin G (immunology) Immunologic Memory Mice Mice, Inbred BALB C Microscopy, Phase-Contrast Operon Plague (genetics, immunology, prevention & control) Plasmids Polymerase Chain Reaction Recombination, Genetic Salmonella Infections, Animal (microbiology) Salmonella typhimurium (genetics) Spleen (microbiology) T-Lymphocytes (immunology) Transferases (genetics) Vaccination Vaccines, Synthetic (administration & dosage, immunology) Yersinia pestis (genetics, immunology)

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