Neuropeptide Y elevates intracellular Ca2+ and evokes noradrenaline release in SH-SY5Y cells.

Abstract

Exposure of human neuroblastoma SH-SY5Y cells to 300 nM neuropeptide Y (NPY) or 1 microM muscarine separately failed to evoke release of [3H]noradrenaline ([3H]NA). Both agonists, however, induced a modest rise in [Ca2+]i. When NPY and muscarine were applied simultaneously, the rise in [Ca2+]i was greater than the sum of the rises of either agonist applied alone, and also evoked [3H]NA release, NPY evoked a rise in [Ca2+]i when applied during prolonged exposure to muscarine, although the peak level of [Ca2+]i was significantly lower (p < 0.05) than that reached following simultaneous application, and [3H]NA release was not stimulated. Simultaneous exposure of SH-SY5Y cells to muscarine and NPY thus induces a greater than additive rise in [Ca2+]i exceeding a critical level required to evoke [3H]NA release.

• Authors: R L McDonald, P F Vaughan, C Peers
• Journal: Neuroreport (Neuroreport) Vol. 7 Issue 18 Pg. 2913-6 (Nov 25 1996) ISSN: 0959-4965 [Print] England
• PMID: 9116208 (Publication Type: Journal Article)

Chemical References

• Muscarinic Agonists
• Neuropeptide Y
• Muscarine
• Calcium
• Norepinephrine

Topics

• Calcium (metabolism)
• Cell Line
• Humans
• Muscarine (pharmacology)
• Muscarinic Agonists (pharmacology)
• Neuropeptide Y (pharmacology)
• Norepinephrine (metabolism)
• Stimulation, Chemical