HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Preparation and biodistribution of copper-67-labeled porphyrins and porphyrin-A6H immunoconjugates.

Abstract
The synthetic porphyrins, N-benzyl-5,10,15,20-tetrakis (4-carboxyphenyl) porphine (N-bzHTCPP) and N-4-nitrobenzyl-5-(4-carboxyphenyl)-10,15,20-tris(4-sulfophenyl) porphine (N-bzHCS3P), represent excellent radiocopper chelating agents that may find utility in antibody-mediated diagnosis and/or therapy. N-bzHCS3P was conjugated to an anti-renal cell carcinoma (RCC) antibody, A6H, and labeled with copper-67. 67CuCS3P-A6H was studied for its biodistribution in human RCC xenograft-bearing nude mice, along with the radiolabeled free porphyrins. The porphyrins resulted in tumor:blood ratios in the range of 3 to 4 after 48 h. The radiolabeled antibody achieved a tumor:blood ratio of over 16 after 45 h, indicating accumulation at the desired site. However, unwanted localization also occurred in the liver and spleen, which will have to be rectified before realizing the full potential of this approach.
AuthorsM K Bhalgat, J C Roberts, J A Mercer-Smith, B D Knotts, R L Vessella, D K Lavallee
JournalNuclear medicine and biology (Nucl Med Biol) Vol. 24 Issue 2 Pg. 179-85 (Feb 1997) ISSN: 0969-8051 [Print] United States
PMID9089710 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Antibodies
  • Chelating Agents
  • Copper Radioisotopes
  • Porphyrins
  • N-benzyl-5,10,15,20-tetrakis(4-carboxyphenyl)porphine
  • N-4-nitrobenzyl-5-(4-carboxyphenyl)-10,15,20-tris(4-sulfophenyl)porphine
Topics
  • Animals
  • Antibodies
  • Biological Transport
  • Carcinoma, Renal Cell (immunology, metabolism)
  • Chelating Agents
  • Copper Radioisotopes (pharmacokinetics)
  • Humans
  • Isotope Labeling
  • Kidney Neoplasms (immunology, metabolism)
  • Male
  • Mice
  • Mice, Nude
  • Porphyrins (pharmacokinetics)
  • Radioimmunodetection
  • Time Factors
  • Tissue Distribution
  • Transplantation, Heterologous
  • Tumor Cells, Cultured

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: