Solubilization and reconstitution of vesicular stomatitis virus envelope using octylglucoside.

Abstract	Reconstituted vesicular stomatitis virus envelopes or virosomes are formed by detergent removal from solubilized intact virus. We have monitored the solubilization process of the intact vesicular stomatitis virus by the nonionic surfactant octylglucoside at various initial virus concentrations by employing turbidity measurements. This allowed us to determine the phase boundaries between the membrane and the mixed micelles domains. We have also characterized the lipid and protein content of the solubilized material and of the reconstituted envelope. Both G and M proteins and all of the lipids of the envelope were extracted by octylglucoside and recovered in the reconstituted envelope. Fusion activity of the virosomes tested either on Vero cells or on liposomes showed kinetics and pH dependence similar to those of the intact virus.
Authors	M Paternostre, M Viard, O Meyer, M Ghanam, M Ollivon, R Blumenthal
Journal	Biophysical journal (Biophys J) Vol. 72 Issue 4 Pg. 1683-94 (Apr 1997) ISSN: 0006-3495 [Print] United States
PMID	9083672 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Detergents Glucosides Liposomes Nucleocapsid Proteins Phosphatidylethanolamines Polystyrenes Rhodamines Viral Envelope Proteins lissamine-rhodamine-phosphatidylethanolamine octyl-beta-D-glucoside Sodium Chloride N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)phosphatidylethanolamine Amberlite XAD-2 resin
Topics	Animals Chlorocebus aethiops Detergents Electrophoresis, Polyacrylamide Gel Fluorescence Glucosides Hydrogen-Ion Concentration Kinetics Liposomes Membrane Fusion Nephelometry and Turbidimetry Nucleocapsid Nucleocapsid Proteins Phosphatidylethanolamines Polystyrenes (metabolism) Rhodamines Sodium Chloride Solubility Vero Cells Vesicular stomatitis Indiana virus (chemistry, physiology) Viral Envelope Proteins (chemistry)

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