Study of apoptosis in human lymphocytes by toxic substances implicated in toxic oil syndrome.

Abstract

Toxic Oil Syndrome is a multisystemic disease that occurred in epidemic proportions in Spain in 1981 caused by the ingestion of rapeseed oil denatured with aniline. Several data implicate T cells in the pathogenesis of the disease. We evaluated the mechanisms of cytotoxicity in human lymphocytes of TOS-related products: aniline, 3-(N-phenylamino)-1,2-propanediol and its mono- and di-oleyl esters and eosinophilia myalgia-related product such as 3-(phenylamino)-L-alanine, which is chemically similar to 3-(N-phenylamino)-1,2-propanediol, and has been found in manufactured L-tryptophan. Our results show that only di-oleyl ester of 3-(N-phenylamino)-1,2-propanediol induces apoptosis in human lymphocytes, in a concentration and time-dependent way, confirmed by morphology, expression of phosphatidylserine in membrane and analysis of DNA degradation.

• Authors: S Gallardo, B Cárdaba, V del Pozo, B de Andrés, I Cortegano, A Jurado, P Tramón, P Palomino, C Lahoz
• Journal: Toxicology (Toxicology) Vol. 118 Issue 1 Pg. 71-82 (Mar 14 1997) ISSN: 0300-483X [Print] Ireland
• PMID: 9074655 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Aniline Compounds
• Coloring Agents
• Fatty Acids, Monounsaturated
• Plant Oils
• Propylene Glycols
• Rapeseed Oil
• 3-(N-phenylamino)-1,2-propanediol 1-oleoyl ester
• Triolein
• 3-(phenylamino)alanine
• 3-phenylamino-1,2-propanediol
• DNA
• 3-(N-phenylamino)-1,2-propanediol 1,2-dioleoyl ester
• Trypan Blue
• Alanine
• aniline

Topics

• Alanine (analogs & derivatives, toxicity)
• Aniline Compounds (toxicity)
• Apoptosis
• Brassica
• Cells, Cultured
• Coloring Agents
• DNA (metabolism)
• Dose-Response Relationship, Drug
• Fatty Acids, Monounsaturated
• Flow Cytometry
• Foodborne Diseases (immunology)
• Humans
• Lymphocytes (cytology, drug effects)
• Microscopy, Fluorescence
• Plant Oils (poisoning)
• Propylene Glycols (toxicity)
• Rapeseed Oil
• Syndrome
• Triolein (toxicity)
• Trypan Blue