| Abstract | Cytokines produced by alveolar macrophages are likely involved in the regulation of the immune response arising from respiratory syncytial virus (RSV) infection. Both infectious and UV-inactivated RSV were effective in inducing BALB/c mouse alveolar macrophages to synthesize increased levels of IL-6 mRNA and secreted IL-6 protein. No increase in IL-1beta (either mRNA or secreted protein) was observed. The augmented production of IL-6 was activated by purified virus and was reduced by pretreating virus with virus-neutralizing antiserum, demonstrating a requirement for virus in the enhanced IL-6 response. The results suggest that the exposure of BALB/c alveolar macrophages to small quantities of RSV (in the absence of detectable virus replication) is sufficient to trigger IL-6 production. The finding that UV-inactivated virus was effective in triggering IL-6 production by mouse alveolar macrophages is similar to that reported in human alveolar macrophages, providing further validation of the BALB/c mouse as a useful animal model for human RSV infection. |
| Authors | A W Stadnyk, T L Gillan, R Anderson
(Affiliation: Departments of Pediatrics, Dalhousie University, Halifax, Nova Scotia, Canada.)
|
| Journal | Cellular immunology
(Cell Immunol)
Vol. 176
Issue 2
Pg. 122-6
(Mar 15 1997)
ISSN: 0008-8749 UNITED STATES |
| PMID | 9073384
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
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| Topics |
- Animals
- Cells, Cultured
- Disease Models, Animal
- Female
- Interleukin-6
(biosynthesis)
- Macrophages, Alveolar
(immunology, metabolism, virology)
- Mice
- Mice, Inbred BALB C
- Respiratory Syncytial Viruses
(immunology)
- Ultraviolet Rays
- Virus Replication
(immunology)
|
