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Apoptotic cell death of human T lymphoblastoid cells induced by arginine deiminase.

Abstract
A growth-inhibitory substance found in the culture of a B-precursor leukemia cell line, NALM-20, was purified from the serum-free culture medium and identified as arginine deiminase derived from Mycoplasma arginini (EC 3.5.3.6). Arginine deiminase strongly inhibited, in a dose-dependent manner, the growth of human T cells and T lymphoblastoid cell lines, but not that of B-precursor and myeloid cell lines. The addition of L-arginine completely restored the growth of T lymphoblastoid cells that had been inhibited by arginine deiminase. The addition of L-ornithine also partially restored it. This enzyme suppressed interleukin-2 (IL-2) production and IL-2 receptor expression in T cells stimulated by non-specific mitogens. The morphologic features of dying cells and DNA fragmentation indicated that arginine deiminase induced apoptotic cell death in T lymphoblasts. Cell cycle analysis revealed that G1-->S transition was blocked in cell treated with arginine deiminase, accompanied by the increase of apoptotic nuclei in the sub-G1 fraction. In conclusion, the deletion of the essential nutrient L-arginine by arginine deiminase significantly inhibited cell growth and activation in T lymphoblasts, accompanied by the induction of apoptotic cell death.
AuthorsY Komada, X L Zhang, Y W Zhou, M Ido, E Azuma
JournalInternational journal of hematology (Int J Hematol) Vol. 65 Issue 2 Pg. 129-41 (Feb 1997) ISSN: 0925-5710 [Print] Japan
PMID9071817 (Publication Type: Journal Article)
Chemical References
  • Hydrolases
  • arginine deiminase
Topics
  • Apoptosis (drug effects)
  • Cell Cycle (drug effects)
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Humans
  • Hydrolases (pharmacology)
  • Mycoplasma (enzymology)
  • T-Lymphocytes (pathology)

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