Binding and transport of melanoma-specific antigenic peptides by the transporter associated with antigen processing.

Abstract

To gain insight into how tumor antigens are generated and presented, a panel of peptides corresponding to melanoma-specific T cell epitopes were tested for their transport capacity by the transporter associated with antigen processing (TAP). The melanoma epitopes exhibited differential capacities to be transported by TAP in streptolysin O-permeabilized cells, as well as differential competition for peptide binding to TAP. The data indicate that some melanoma-specific epitopes are good substrates for TAP, while others are poor substrates for TAP. One of the epitopes, derived from tyrosinase, was transported into the endoplasmic reticulum (ER), in spite of being a poor competitor for reporter peptide transport and for peptide binding. These results suggest that the melanoma antigens follow distinct pathways for presentation, along the MHC class I pathway.

• Authors: S A Chang, V G Lacaille, D S Guttoh, M J Androlewicz
• Journal: Molecular immunology (Mol Immunol) Vol. 33 Issue 15 Pg. 1165-9 (Oct 1996) ISSN: 0161-5890 [Print] England
• PMID: 9070664 (Publication Type: Journal Article)

Chemical References

• ATP Binding Cassette Transporter, Subfamily B, Member 3
• ATP-Binding Cassette Transporters
• Antigens, Neoplasm
• Epitopes
• Peptides
• TAP2 protein, human

Topics

• ATP Binding Cassette Transporter, Subfamily B, Member 3
• ATP-Binding Cassette Transporters (immunology)
• Amino Acid Sequence
• Antigen Presentation
• Antigens, Neoplasm (metabolism)
• Epitopes
• Humans
• Major Histocompatibility Complex (immunology)
• Melanoma (immunology)
• Peptides (chemistry)
• Protein Binding