Abstract | BACKGROUND: AIM: METHODS AND RESULTS: Hu-related molecules of 35 to 41 kDa were detected in the membrane of small-cell lung cancers and neuroblastomas using: (1) immunofluorescence, (2) absorption assays, (3) Western blotting on membrane fractions, and (4) surface biotinylation. The antibodies recognizing these membrane components were specifically absorbed by recombinant HuD protein. There was a perfect correlation between nuclear and membrane Hu expression. To determine the purity of the subcellular fractions, their reactivity with antibodies recognizing the A2 nuclear ribonucleoprotein and the cytoplasmic mitogen-activated protein kinase was examined. None of them was detected in the membrane fractions reactive with sera containing Hu antibodies. CONCLUSIONS: IMPLICATIONS:
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Authors | M Torà, F Graus, C de Bolòs, F X Real |
Journal | Neurology
(Neurology)
Vol. 48
Issue 3
Pg. 735-41
(Mar 1997)
ISSN: 0028-3878 [Print] United States |
PMID | 9065557
(Publication Type: Clinical Trial, Controlled Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Surface
- Autoantibodies
- ELAV Proteins
- ELAV-Like Protein 4
- ELAVL4 protein, human
- Immunoglobulin G
- Nerve Tissue Proteins
- RNA-Binding Proteins
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Topics |
- Antigens, Surface
(analysis)
- Autoantibodies
(immunology)
- Blotting, Western
- Carcinoma, Small Cell
(immunology)
- Cell Nucleus
(chemistry)
- Cytoplasm
(chemistry)
- ELAV Proteins
- ELAV-Like Protein 4
- Encephalomyelitis
(immunology)
- Humans
- Immunoglobulin G
(immunology)
- Lung Neoplasms
(immunology)
- Nerve Tissue Proteins
- Neuroblastoma
(immunology)
- RNA-Binding Proteins
(analysis)
- Tumor Cells, Cultured
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