Expression of exogenous NF-IL6 induces apoptosis in Sp2/0-Ag14 myeloma cells.

Abstract

NF-IL6 (or C/EBP beta) is a multifunctional transcription factor of the C/EBP family. To study the effects of exogenous expression of NF-IL6 on the growth of cells of hematopoietic tumors, we infected SP2/0-Ag14 myeloma cells with pLXSN recombinant retrovirus harboring the NF-IL6 cDNA. We found that the transfected cells revealed changes typical of the apoptosis and finally died. The expression of NF-IL6 protein was shown by a "supershift" assay in the NF-IL6-transfected cells. In controls, where cells were infected by the retroviral vector with a mutant NF-IL6 coding region as well as the vector without insert, no sign for expression of NF-IL6 and apoptosis was observed. Therefore, our work shows that the apoptosis of this myeloma cell line was induced by the exogenous NF-IL6 expression. This is significantly different from the apparent effects of NF-IL6 in leukemia cells and in fibroblasts. These facts indicate that NF-IL6 exerts different functions in different cell types. We discuss possible mechanisms for the NF-IL6-induced apoptosis.

• Authors: M S Zhu, D G Liu, H Q Cheng, X Y Xu, Z P Li
• Journal: DNA and cell biology (DNA Cell Biol) Vol. 16 Issue 2 Pg. 127-35 (Feb 1997) ISSN: 1044-5498 [Print] United States
• PMID: 9052733 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CCAAT-Enhancer-Binding Proteins
• DNA, Neoplasm
• DNA-Binding Proteins
• Nuclear Proteins
• Transcription Factors

Topics

• Animals
• Apoptosis (physiology)
• CCAAT-Enhancer-Binding Proteins
• Cell Division
• Cell Nucleus (pathology)
• DNA, Neoplasm (analysis)
• DNA-Binding Proteins (genetics, physiology)
• Gene Expression
• Genetic Vectors (genetics)
• Mice
• Multiple Myeloma (metabolism, pathology)
• Mutation
• Nuclear Proteins (genetics, physiology)
• Retroviridae (genetics)
• Transcription Factors (genetics, physiology)
• Tumor Cells, Cultured