Induction of human cytotoxic T lymphocytes specific for prostate-specific antigen.

Abstract

Prostate-specific antigen (PSA), a tissue-specific protein expressed by most adenocarcinomas of the prostate, might be a useful target for T-cell-mediated immunotherapy of prostate cancers. The current study examined whether it is possible to elicit human cytotoxic T lymphocytes (CTL) with specificity for PSA. A synthetic nonamer peptide, corresponding to residues 146-154 of PSA and containing a canonical HLA-A2-binding motif, was shown to stabilize the expression of HLA-A2 on the T2 antigen-processing mutant cell line. Repeated in vitro stimulation of peripheral blood lymphocytes from a normal HLA-A2+ donor induced CTL with specificity for the PSA 146-154 peptide. The peptide-induced CTL expressed the CD4- CD8+ cell surface phenotype and were restricted by HLA-A2. A large portion of patients with prostate cancer express the HLA-A2 phenotype, implying that many prostate cancers might be targeted by HLA-A2-restricted CTL with specificity for the PSA 146-154 epitope.

• Authors: B H Xue, Y Zhang, J A Sosman, D J Peace
• Journal: The Prostate (Prostate) Vol. 30 Issue 2 Pg. 73-8 (Feb 01 1997) ISSN: 0270-4137 [Print] United States
• PMID: 9051144 (Publication Type: Journal Article)

Chemical References

• Epitopes
• HLA-A2 Antigen
• Peptide Fragments
• Peptides
• prostate-specific antigen (146-154)
• Prostate-Specific Antigen

Topics

• Adenocarcinoma (immunology, therapy)
• Epitopes (chemistry, immunology)
• HLA-A2 Antigen (immunology)
• Humans
• Immunity, Cellular
• Immunization
• Immunotherapy
• Male
• Peptide Fragments (chemistry, immunology)
• Peptides (chemistry, immunology)
• Prostate-Specific Antigen (chemistry, immunology)
• Prostatic Neoplasms (immunology, therapy)
• T-Lymphocytes, Cytotoxic (immunology)
• Tumor Cells, Cultured