HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Sequence-specific interactions of minor groove binders with the 154 base pair HindIII-RsaI restriction fragment of cDNA of the human Tau 40 protein involved in pathology of Alzheimer's disease.

Abstract
The DNA minor groove binders netropsin, distamycin and four structurally related bisquaternary ammonium heterocycles (BQA), SN 6999, SN 6570, SN 6132 and SN 6131, were investigated for sequence-specific interactions with the 154 base pair fragment of cDNA of the human Tau 40 protein (h Tau 40 protein), involved in pathology of Alzheimer's disease. The base sequences 5' AATCTT 3', 5' AATATT 3' and 5' TTTCAATCTTTTTATTT 3' were identified as ligand specific binding sites and demonstrate the obvious dA.dT binding preference. Footprinting titration experiments were performed to estimate sequence-specific binding constants (KA). The KA-values were in the order of 10(6)M-1 and dependent on DNA base sequence as well as ligands used. The highest values estimated were for netropsin (KA = 5.0 x 10(6)M-1) and the quinoline derivative SN 6999 (KA = 6.2 x 10(6)M-1) binding to the sequence 5' ATAAT 3'. Microscopic binding constants are determined by the base sequence rather than by the length of dA.dT stretches. In the extended dA.dT run, 5' TTTCAATCTTTTTATTT 3', netropsin and distamycin binding tolerates the presence of two dG.dC base pairs, as indicated by nearly unaffected footprints. In contrast, the failure of BQAs to form footprints demonstrates their significantly decreased binding selectivity.
AuthorsL Kittler, D Matesoi, A Bell, B C Baguley, E Unger, G Löber
JournalBiochemistry and molecular biology international (Biochem Mol Biol Int) Vol. 41 Issue 1 Pg. 143-52 (Jan 1997) ISSN: 1039-9712 [Print] AUSTRALIA
PMID9043643 (Publication Type: Journal Article)
Chemical References
  • DNA, Complementary
  • Distamycins
  • Ligands
  • Oligopeptides
  • Pyridinium Compounds
  • Quinolinium Compounds
  • tau Proteins
  • SN 6131
  • SN 6132
  • Netropsin
  • stallimycin
  • SN 6999
  • Deoxyribonuclease HindIII
  • Deoxyribonucleases, Type II Site-Specific
  • GTAC-specific type II deoxyribonucleases
Topics
  • Alzheimer Disease (genetics)
  • Base Sequence
  • Binding Sites
  • DNA Footprinting
  • DNA, Complementary (metabolism)
  • Deoxyribonuclease HindIII
  • Deoxyribonucleases, Type II Site-Specific
  • Distamycins (metabolism)
  • Humans
  • Kinetics
  • Ligands
  • Molecular Sequence Data
  • Netropsin (metabolism)
  • Oligopeptides (metabolism)
  • Pyridinium Compounds (metabolism)
  • Quinolinium Compounds (metabolism)
  • tau Proteins (genetics)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: