Manganese-dependent Dopa/tyrosine sulfation in HepG2 human hepatoma cells: novel Dopa/tyrosine sulfotransferase activities associated with the human monoamine-form phenol sulfotransferase.

Abstract

Human monoamine (M)-form phenol sulfotransferase (PST) was PCR-cloned and transiently expressed in COS-7 cells. The recombinant enzyme was demonstrated to display not only the previously reported sulfotransferase activity toward dopamine, but also novel manganese-dependent Dopa/tyrosine sulfotransferase activities. These results imply a new functional role of the human M-form PST in the homeostatic regulation of Dopa and tyrosine.

• Authors: Y Sakakibara, J Katafuchi, Y Takami, T Nakayama, M Suiko, H Nakajima, M C Liu
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 1355 Issue 2 Pg. 102-6 (Feb 04 1997) ISSN: 0006-3002 [Print] Netherlands
• PMID: 9042329 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Recombinant Proteins
• Manganese
• Dopa-tyrosine sulfotransferase
• Sulfotransferases
• Arylsulfotransferase
• Deoxyribonuclease EcoRI

Topics

• Animals
• Arylsulfotransferase (metabolism)
• COS Cells
• Deoxyribonuclease EcoRI
• Humans
• Manganese (metabolism, pharmacology)
• Recombinant Proteins (metabolism)
• Sequence Homology, Nucleic Acid
• Sulfotransferases (metabolism)
• Tumor Cells, Cultured