Abstract |
Experimental allergic neuritis (EAN) was induced in guinea-pigs by intradermal injection of rabbit peripheral nerve emulsified in Freund's adjuvant. Both sciatic nerves were obtained between 12--24 hr after clinical symptoms were evident. Several fascicles from each nerve were isolated for histochemical studies with NADH-diaphorase ( NADH) and acid phosphatase (AP) applied to teased nerve fibres. Small pieces were processed for electron microscopy, and a fascicle was teased after staining with osmium tetroxide. In isolated nerve fibres stained with histochemical techniques myelin lesions of segmental character were found closely related to inflammatory cells; Schwann cell cytoplasm in contact with mononuclear cells showing a heavy enzymatic activity ( NADH and AP). Under polarized light, the underliying myelin showed focal loss of birefringence. Some electron-microscopic pictures suggested active myelin breakdown by mononuclear cells. The possibility of primary Schwann cell damage by mononuclear cells with subsequent demyelination is discussed.
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Authors | A Martinez, A Blanes, S Ramon y Cajal Junquera, I De Ispizua, C Campo |
Journal | Journal of the neurological sciences
(J Neurol Sci)
Vol. 33
Issue 1-2
Pg. 61-9
(Aug 1977)
ISSN: 0022-510X [Print] Netherlands |
PMID | 903790
(Publication Type: Journal Article)
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Chemical References |
- Dihydrolipoamide Dehydrogenase
- Acid Phosphatase
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Topics |
- Acid Phosphatase
(metabolism)
- Animals
- Dihydrolipoamide Dehydrogenase
(metabolism)
- Female
- Guinea Pigs
- Histocytochemistry
- Hypersensitivity
(enzymology, pathology)
- Neuritis
(enzymology, pathology)
- Schwann Cells
(enzymology, ultrastructure)
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