Murine cytomegalovirus inactivated by sodium periodate is innocuous and immunogenic in mice and protects them against death and infection.

Abstract

Sodium periodate (10 mM, 4 degrees C) inactivated murine cytomegalovirus (MCMV) very rapidly (loss of 2 to 3 log of viral infectivity per minute). Periodate-treated MCMV (PI-MCMV) was shown to be innocuous in mice, as determined by the inability of the virus to replicate. PI-MCMV induced a strong humoral immune response, with a high level of neutralizing antibodies. Mice immunized with PI-MCMV were protected against death and infection, when a lethal challenge with the virulent virus was administered 3 weeks after immunization and from death but not infection when virulent virus was administered at 3 months. Finally, no reactivation of potentially latent challenge virus (sublethal dose at 3 weeks) was observed in animals immunosuppressed at 6 months after immunization. Taken together, these results suggest that periodate could serve as an inactivating agent to prepare killed vaccines.

• Authors: F Geoffroy, N Moachon, J Rodwell, G A Quash
• Journal: Vaccine (Vaccine) Vol. 14 Issue 17-18 Pg. 1686-94 (Dec 1996) ISSN: 0264-410X [Print] Netherlands
• PMID: 9032900 (Publication Type: Journal Article)

Chemical References

• Antibodies, Viral
• Vaccines, Inactivated
• Periodic Acid
• metaperiodate

Topics

• Animals
• Antibodies, Viral (biosynthesis)
• Cells, Cultured
• Cytomegalovirus Infections (prevention & control)
• Female
• Immunization
• Kinetics
• Mice
• Mice, Inbred BALB C
• Muromegalovirus (drug effects, immunology, pathogenicity)
• Periodic Acid
• Vaccines, Inactivated
• Virulence
• Virus Latency
• Virus Replication