Glucose-responsitivity and expression of an ATP-stimulatable, Ca(2+)-independent phospholipase A2 enzyme in clonal insulinoma cell lines.

Abstract

We have previously reported that pancreatic islet beta-cells and clonal HIT insulinoma cells express an ATP-stimulatable Ca(2+)-independent phospholipase A2 (ASCI-PLA2) enzyme and that activation of this enzyme appears to participate in glucose-stimulated insulin secretion. To further examine this hypothesis, glucose-responsitivity and expression of ASCI-PLA2 activity in various insulinoma cell lines were examined. Secretagogue-stimulated insulin secretion was observed with beta TC6-f7 and early passage (EP)-beta TC6 cells. In contrast, RIN-m5f, beta TC3, and late passage (LP)-beta TC6 cells exhibited little secretagogue-induced secretion. A haloenollactone suicide substrate (HELSS) which inhibits ASCI-PLA2 activity ablated secretagogue-induced insulin secretion from beta TC6-f7 and EP-beta TC6 cells. All insulinoma cell lines studied expressed both cytosolic and membrane-associated Ca(2+)-independent PLA2 activities which were inhibited by HELSS. The cytosolic enzymatic activity in the glucose-responsive beta TC6-f7 and EP-beta TC6 cells was activated by ATP and protected against thermal denaturation by ATP, but this was not the case in the glucose-unresponsive RIN-m5f, beta TC3, or LP-beta TC6 cells. Comparison of the distribution of Ca(2+)-independent PLA2 activity revealed that membrane-associated activity was higher than cytosolic activity in beta TC6-f7 and EP-beta TC6 cells but not in RIN-m5f, beta TC3, or LP-beta TC6 cells. Insensitivity of cytosolic activity to ATP may prevent association of the PLA2 activity with membrane substrates and contribute to attenuated glucose-responsitivity in the RIN-m5f, beta TC3, or LP-beta TC6 cells. HIT insulinoma cells were also found to undergo a decline in both glucose-responsitivity and membrane-associated Ca(2+)-independent PLA2 activity upon serial passage in culture, and this was associated with a reduction in membrane content of arachidonate-containing phospholipids. These and previous results suggest that the ATP-stimulatable PLA2 enzyme may participate in glucose-induced insulin secretion.

• Authors: S Ramanadham, M J Wolf, B Li, A Bohrer, J Turk
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 1344 Issue 2 Pg. 153-64 (Jan 21 1997) ISSN: 0006-3002 [Print] Netherlands
• PMID: 9030192 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Insulin
• Adenosine Triphosphate
• Phospholipases A
• Phospholipases A2
• Glucose
• Calcium

Topics

• Adenosine Triphosphate (pharmacology)
• Animals
• Calcium (pharmacology)
• Cell Membrane (enzymology)
• Cricetinae
• Cytosol (enzymology)
• Enzyme Activation (drug effects)
• Glucose (pharmacology)
• Insulin (metabolism)
• Insulin Secretion
• Insulinoma (enzymology, metabolism)
• Islets of Langerhans (drug effects, enzymology, metabolism)
• Mice
• Pancreatic Neoplasms (enzymology, metabolism)
• Phospholipases A (metabolism)
• Phospholipases A2
• Rats
• Tumor Cells, Cultured