Abstract |
The lymphotoxin beta receptor (LT beta R) was originally described as a transcribed sequence encoded on human chromosome 12p, with homology to the TNF receptor family. Subsequently, a recombinant LT beta R was shown to bind LT alpha LT beta heteromeric complexes. In this study, we have shown that LT beta R is expressed in a variety of tissues and cell lines of monocytic lineage, as well as in fibroblast and human melanoma cell lines. Unlike other members of the TNF receptor family, LT beta R is not expressed by peripheral blood T cells. A chimeric fusion protein consisting of the extracellular domain of LT beta R fused to the Fc region of human IgG1 was used to develop mAbs against LT beta R. Cross-linking LT beta R on A375 melanoma cells with these Abs generated an antiproliferative signal. In addition, the IL-8 and RANTES chemokines, early indicators of inflammation, were secreted by the A375 melanoma line and the WI38VA13 fibroblast line in response to cross-linking of LT beta R. These same activities could be induced by membrane-bound and soluble LT beta and LT alpha LT beta oligomers.
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Authors | M A Degli-Esposti, T Davis-Smith, W S Din, P J Smolak, R G Goodwin, C A Smith |
Journal | Journal of immunology (Baltimore, Md. : 1950)
(J Immunol)
Vol. 158
Issue 4
Pg. 1756-62
(Feb 15 1997)
ISSN: 0022-1767 [Print] United States |
PMID | 9029113
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Monoclonal
- Biopolymers
- Chemokines
- Cross-Linking Reagents
- Growth Inhibitors
- Immunoglobulin Fc Fragments
- LTB protein, human
- LTBR protein, human
- Lymphotoxin beta Receptor
- Lymphotoxin-alpha
- Lymphotoxin-beta
- Membrane Proteins
- Receptors, Tumor Necrosis Factor
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Topics |
- Adult
- Antibodies, Monoclonal
(biosynthesis)
- Biopolymers
- Chemokines
(biosynthesis)
- Cross-Linking Reagents
- Fetus
- Growth Inhibitors
(metabolism, physiology)
- Humans
- Immunoglobulin Fc Fragments
(biosynthesis)
- Lymphotoxin beta Receptor
- Lymphotoxin-alpha
(immunology, metabolism, physiology)
- Lymphotoxin-beta
- Melanoma
(immunology, metabolism, pathology)
- Membrane Proteins
(immunology, metabolism, pharmacology, physiology)
- Receptors, Tumor Necrosis Factor
(immunology, metabolism, physiology)
- Solubility
- Tissue Distribution
- Tumor Cells, Cultured
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