Abstract |
The antitumor effect of the combined administration with recombinant human interleukin-2 (rIL-2) and sizofiran (SPG), a single glucan of Shizophyllum commune Fries, was studied in vivo in C57BL/6 mice intraperitoneally inoculated with EL-4 lymphoma. The effect was evaluated by a) comparing the survival time of the mice, b) analysis of the intraperitoneal cell population in Giemsa-stained specimens, c) surface marker analysis of peritoneal exudative cells with flow cytometry, d) cytotoxic assay of cells against EL-4 and Yac-1 lymphoma, and e) elimination of some cell populations by monoclonal antibodies, to identify the antitumor-effector cells showing cytotoxic activity. The survival of mice given both rIL-2 and SPG was significantly longer than the control mice or those given SPG alone or rIL-2 alone. It was demonstrated that the administration of SPG and/or rIL-2 to the EL-4 lymphoma-bearing mice activated immune-response cells in the peritoneal cavity such as T lymphocytes, NK cells, or macrophages, which might be effective in reducing lymphoma cells. The combination of rIL-2 and SPG administration appears to activate the antitumor-immune response at the tumor site more effectively than when either agent was administered alone.
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Authors | Y Kano, H Kakuta, J Hashimoto |
Journal | Biotherapy (Dordrecht, Netherlands)
(Biotherapy)
Vol. 9
Issue 4
Pg. 241-7
( 1996)
ISSN: 0921-299X [Print] Netherlands |
PMID | 9012543
(Publication Type: Journal Article)
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Chemical References |
- Antibodies, Monoclonal
- Interleukin-2
- Recombinant Proteins
- Sizofiran
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Topics |
- Animals
- Antibodies, Monoclonal
(immunology)
- Antineoplastic Combined Chemotherapy Protocols
(therapeutic use)
- Cytotoxicity, Immunologic
- Flow Cytometry
- Humans
- Interleukin-2
(administration & dosage)
- Lymphoma
(immunology, mortality, therapy)
- Mice
- Mice, Inbred C57BL
- Recombinant Proteins
(administration & dosage)
- Sizofiran
(administration & dosage)
- Survival Rate
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