Abstract |
The C-terminal region of parathyroid hormone-related protein ( PTHrP) containing the sequence (107-111) appears to be a potent inhibitor of osteoclastic bone resorption. In the present study, we have investigated the effect of human (h) PTHrP (107-139) and hPTHrP (107-111)NH2 on the proliferation of osteoblastic rat osteosarcoma UMR 106 cells. We found that both C-terminal PTHrP peptides, like hPTHrP (1-141), were antimitogenic for these cells, between 1 pM and 10 nM. [Tyr34]hPTHrP (1-34)NH2 was as potent as these peptides but less effective as growth inhibitor in these cells. UMR 106 cells were found to produce and secrete immunoreactive PTHrP. Addition of anti- PTHrP neutralizing antibodies to C- and N-terminal epitopes of PTHrP increased the growth of these cells. Our data suggest that the antiproliferative effect of these C-terminal PTHrP analogs may be independent of cyclic adenosine 3':5'-monophosphate (cAMP) and mediated by protein kinase C. These findings support an autocrine role of PTHrP in bone metabolism.
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Authors | A Valín, A García-Ocaña, F De Miguel, J L Sarasa, P Esbrit |
Journal | Journal of cellular physiology
(J Cell Physiol)
Vol. 170
Issue 2
Pg. 209-15
(Feb 1997)
ISSN: 0021-9541 [Print] United States |
PMID | 9009150
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Growth Inhibitors
- PTHLH protein, human
- Parathyroid Hormone
- Parathyroid Hormone-Related Protein
- Peptide Fragments
- Proteins
- parathyroid hormone-related protein (107-111)
- Teriparatide
- parathyroid hormone-related protein (1-141)
- parathyroid hormone-related protein (107-139)
- Cyclic AMP-Dependent Protein Kinases
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Topics |
- Animals
- Bone Neoplasms
- Cell Division
(drug effects)
- Cell Line
- Cyclic AMP-Dependent Protein Kinases
(metabolism)
- Growth Inhibitors
(pharmacology)
- Humans
- Osteoblasts
(cytology, drug effects)
- Osteosarcoma
- Parathyroid Hormone
(biosynthesis, pharmacology)
- Parathyroid Hormone-Related Protein
- Peptide Fragments
(pharmacology)
- Protein Biosynthesis
- Proteins
(pharmacology)
- Rats
- Teriparatide
(pharmacology)
- Tumor Cells, Cultured
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