Abstract |
A cultured cell line was derived from a hemangiopericytoma obtained at surgery from a 9 year-old boy with hypophosphatemic rickets. Hypophosphatemia and metabolic bone disease were cured in the patient after tumor removal. Cells from passage 5 were transplanted into 8 week-old athymic female mice. Additional animals received innocula of cells thawed from the stored original tumor tissue, as well as MRC-5 fibroblasts. Serum phosphate levels 3-7 months post- transplantation were lowest in mice which received the cultured cell line (n = 5) 6.1 +/- 0.6 mg/dl (p < 0.05 compared to the other groups), thawed tumor cells (n = 8) 7.2 +/- 0.7 mg/dl, MRC fibroblasts (n = 4) 8.1 +/- 1.0 mg/dl, no transplant (n = 10) 8.7 +/- 1.9 mg/dl. Repeat of the experiment with cultured tumor cells from passage 12 no longer altered phosphate levels. A substance produced and release by the tumor in situ and by tumor-derived cultured cells is capable of producing hypophosphatemia. Experimental manipulation of functional tumor-derived cell lines may help elucidate the factor(s) causing hypophosphatemia in oncogenic osteomalacia/ rickets.
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Authors | S A Chalew, J C Lovchik, C M Brown, C C Sun |
Journal | Journal of pediatric endocrinology & metabolism : JPEM
(J Pediatr Endocrinol Metab)
1996 Nov-Dec
Vol. 9
Issue 6
Pg. 593-7
ISSN: 0334-018X [Print] Germany |
PMID | 9004174
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Animals
- Child
- Desmin
(analysis)
- Female
- Hemangiopericytoma
(complications)
- Humans
- Hypophosphatemia
(etiology)
- Hypophosphatemia, Familial
(etiology)
- Male
- Mice
- Mice, Nude
- Neoplasm Transplantation
- Tumor Cells, Cultured
- Vimentin
(analysis)
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