Hypophosphatemia induced in mice by transplantation of a tumor-derived cell line from a patient with oncogenic rickets.

Abstract

A cultured cell line was derived from a hemangiopericytoma obtained at surgery from a 9 year-old boy with hypophosphatemic rickets. Hypophosphatemia and metabolic bone disease were cured in the patient after tumor removal. Cells from passage 5 were transplanted into 8 week-old athymic female mice. Additional animals received innocula of cells thawed from the stored original tumor tissue, as well as MRC-5 fibroblasts. Serum phosphate levels 3-7 months post-transplantation were lowest in mice which received the cultured cell line (n = 5) 6.1 +/- 0.6 mg/dl (p < 0.05 compared to the other groups), thawed tumor cells (n = 8) 7.2 +/- 0.7 mg/dl, MRC fibroblasts (n = 4) 8.1 +/- 1.0 mg/dl, no transplant (n = 10) 8.7 +/- 1.9 mg/dl. Repeat of the experiment with cultured tumor cells from passage 12 no longer altered phosphate levels. A substance produced and release by the tumor in situ and by tumor-derived cultured cells is capable of producing hypophosphatemia. Experimental manipulation of functional tumor-derived cell lines may help elucidate the factor(s) causing hypophosphatemia in oncogenic osteomalacia/rickets.

• Authors: S A Chalew, J C Lovchik, C M Brown, C C Sun
• Journal: Journal of pediatric endocrinology & metabolism : JPEM (J Pediatr Endocrinol Metab) 1996 Nov-Dec Vol. 9 Issue 6 Pg. 593-7 ISSN: 0334-018X [Print] Germany
• PMID: 9004174 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Desmin
• Vimentin

Topics

• Animals
• Child
• Desmin (analysis)
• Female
• Hemangiopericytoma (complications)
• Humans
• Hypophosphatemia (etiology)
• Hypophosphatemia, Familial (etiology)
• Male
• Mice
• Mice, Nude
• Neoplasm Transplantation
• Tumor Cells, Cultured
• Vimentin (analysis)