Complete amino-acid sequence of the beta-subunit of VTX from venom of the stonefish (Synanceia verrucosa) as identified from cDNA cloning experiments.

Abstract

A cDNA encoding a subunit of the verrucotoxin (VTX) has been identified from a cDNA library derived from stonefish venom glands. It encodes a polypeptide of 708 amino-acid residues, followed by a 3'-untranslated region of 895 bp long. The ORF contains the complete mature sequence of the beta-subunit of the VTX, as inferred from both the presence of an identical N-terminus sequence and 96% homology among the 506 amino terminus residues found in the partial sequence of the beta-subunit of the stonustoxin from Synanceia horrida (Ghadessy, F.J., Jeyaseelan, K., Chung, M.C.M., Khoo, H.E., and Yuen, R. (1994) Toxicon 32, 1684-1688). Upstream the mature sequence, we noticed the presence of an incomplete peptide of a 13 amino acids, whose unusual primary structure supports the idea of the existence of a propeptide and/or of a new secretion signal.

• Authors: P Garnier, F Ducancel, T Ogawa, J C Boulain, F Goudey-Perrière, C Perrière, A Ménez
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 1337 Issue 1 Pg. 1-5 (Jan 04 1997) ISSN: 0006-3002 [Print] Netherlands
• PMID: 9003430 (Publication Type: Comparative Study, Journal Article)

Chemical References

• DNA, Complementary
• Fish Venoms
• Glycoproteins
• verrucotoxin protein, Synanceia verrucosa

Topics

• Amino Acid Sequence
• Animals
• Base Sequence
• Cloning, Molecular
• DNA, Complementary (genetics)
• Fish Venoms (genetics)
• Fishes (genetics)
• Glycoproteins (genetics)
• Molecular Sequence Data
• Sequence Analysis, DNA
• Sequence Homology, Amino Acid
• Species Specificity