[Comparative study of the synthesis and specificity of enterotoxin from Vibrio cholerae O139 serotype using monoclonal antibodies].

Abstract

Strains of V. cholerae serovar 0139 showed a higher intensity of multiplication and lesser nutritive requirements and produced 2-5 times higher amounts of enterotoxin during in-depth culturing than cholera vibrios of groups O1 and non-O1. R-forms of V. cholerae were characterized by the highest production of toxin. Dot-immunoanalysis and immunoblotting with monoclonal antibodies demonstrated the identity of cholera toxin and enterotoxin of V. cholerae serovar O139. The authors come to a conclusion that selective efficacy of the developed vaccines against diarrhea caused by V. cholerae serovar O139 depends on the presence of homologous O-antigen in their composition.

• Authors: V A Fedorova, T V Alenkina, O V Gromova, E L Devdariani, M N Dzhaparidze, O M Kosmoenko
• Journal: Molekuliarnaia genetika, mikrobiologiia i virusologiia (Mol Gen Mikrobiol Virusol) 1996 Jul-Sep Issue 3 Pg. 3-6 ISSN: 0208-0613 [Print] Russia (Federation)
• Vernacular Title: Sravnitel'noe izuchenie osobennosteĭ sinteza i spetsifichnosti énterotoksina Vibrio cholerae O139 serovara s pomoshch'iu monoklonal'nykh antitel.
• PMID: 8999316 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Antibodies, Monoclonal
• Enterotoxins
• stN protein, Vibrio cholerae

Topics

• Antibodies, Monoclonal
• Antibody Specificity
• Enterotoxins (biosynthesis, immunology)
• Immunoblotting
• Serotyping
• Vibrio cholerae (classification, growth & development, metabolism)