Neuropeptide Y inhibits depolarization-stimulated catecholamine synthesis in rat pheochromocytoma cells.

Abstract

In PC12 rat pheochromocytoma cells differentiated with nerve growth factor (NGF), neuropeptide Y inhibited depolarization-stimulated catecholamine synthesis as determined by in situ measurement of 3,4-dihydroxyphenylalanine (DOPA) production in the presence of the decarboxylase inhibitor m-hydroxybenzylhydrazine (NSD-1015). The inhibition by neuropeptide Y was concentration-dependent and was prevented by pretreatment with pertussis toxin, suggesting the involvement of a GTP-binding protein of the Gi or Go subtype. The neuropeptide Y analog [Leu31,Pro34]neuropeptide Y also caused inhibition of DOPA production, but was less potent than neuropeptide Y itself, while peptide YY and neuropeptide Y-(13-36) had no significant effect. This pattern is most consistent with the involvement of the neuropeptide Y Y3 receptor subtype. In PC12 cells differentiated with dexamethasone, neuropeptide Y also caused a concentration-dependent inhibition of DOPA production, while peptide YY was again without effect. Neuropeptide Y had no effect on DOPA production in undifferentiated PC12 cells. These results indicate that neuropeptide Y can modulate catecholamine synthesis in addition to its modulatory effects on catecholamine release.

• Authors: L A McCullough, T C Westfall
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 287 Issue 3 Pg. 271-7 (Dec 20 1995) ISSN: 0014-2999 [Print] Netherlands
• PMID: 8991801 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Aromatic Amino Acid Decarboxylase Inhibitors
• Gastrointestinal Hormones
• Hydrazines
• Nerve Growth Factors
• Neuropeptide Y
• Peptide Fragments
• Peptides
• Virulence Factors, Bordetella
• neuropeptide Y (13-36)
• Peptide YY
• neuropeptide Y, Leu(31)-Pro(34)-
• Dihydroxyphenylalanine
• Dexamethasone
• 3-hydroxybenzylhydrazine
• Pertussis Toxin
• GTP-Binding Proteins

Topics

• Adrenal Gland Neoplasms (parasitology)
• Analysis of Variance
• Animals
• Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
• Aromatic Amino Acid Decarboxylase Inhibitors
• Cell Differentiation (drug effects)
• Dexamethasone (pharmacology)
• Dihydroxyphenylalanine (metabolism)
• Dose-Response Relationship, Drug
• GTP-Binding Proteins (metabolism)
• Gastrointestinal Hormones (pharmacology)
• Hydrazines (pharmacology)
• Nerve Growth Factors (pharmacology)
• Neuropeptide Y (analogs & derivatives, pharmacology)
• PC12 Cells (cytology, drug effects)
• Peptide Fragments (pharmacology)
• Peptide YY
• Peptides (pharmacology)
• Pertussis Toxin
• Pheochromocytoma (pathology)
• Rats
• Structure-Activity Relationship
• Virulence Factors, Bordetella (pharmacology)