2-Naphthoate catabolic pathway in Burkholderia strain JT 1500.

Abstract

Burkholderia strain (JT 1500), able to use 2-naphthoate as the sole source of carbon, was isolated from soil. On the basis of growth characteristics, oxygen uptake experiments, enzyme assays, and detection of intermediates, a degradation pathway of 2-naphthoate is proposed. The features of this pathway are convergent with those for phenanthrene. We propose a pathway for the conversion of 2-naphthoate to 1 mol (each) of pyruvate, succinate, and acetyl coenzyme A and 2 mol of CO2. During growth in the presence of 2-naphthoate, six metabolites were detected by thin-layer chromatography, high-performance liquid chromatography, and spectroscopy. 1-Hydroxy-2-naphthoate accumulated in the culture broth during growth on 2-naphthoate. Also, the formation of 2'-carboxybenzalpyruvate, phthalaldehydate, phthalate, protocatechuate, and beta-carboxy-cis,cis-muconic acid was demonstrated. (1R,2S)-cis-1,2-Dihydro-1,2-dihydroxy-2-naphthoate was thus considered an intermediate between 2-naphthoate and 1-hydroxy-2-naphthoate, but it was not transformed by whole cells or their extracts. We conclude that this diol is not responsible for the formation of 1-hydroxy-2-naphthoate from 2-naphthoate but that one of the other three diastereomers is not eliminated as a potential intermediate for a dehydration reaction.

• Authors: B Morawski, R W Eaton, J T Rossiter, S Guoping, H Griengl, D W Ribbons
• Journal: Journal of bacteriology (J Bacteriol) Vol. 179 Issue 1 Pg. 115-21 (Jan 1997) ISSN: 0021-9193 [Print] United States
• PMID: 8981987 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Naphthalenes
• Carbon
• 2-naphthoic acid

Topics

• Biodegradation, Environmental
• Burkholderia (enzymology, metabolism)
• Carbon (metabolism)
• Models, Chemical
• Naphthalenes (metabolism)
• Oxygen Consumption