Signaling effects of alpha-thrombin and SFLLRN in rat glioma C6 cells.

Abstract

Effects of thrombin on brain cells, including change of neurite outgrowth and astrocyte shape, are described, but the molecular mechanisms are unclear. We investigated the effects of human alpha-thrombin and a six amino acid thrombin receptor activating peptide (TRAP-6, SFLLRN) on [Ca2+]i, phosphoinositide hydrolysis, and protein kinase C in rat glioma C6 cells. Stimulation of C6 cells with both alpha-thrombin and TRAP-6 resulted in [Ca2+]i mobilization, [3H]Inositol phosphate response, and enhanced immunoreactivity of the protein kinase C (PKC) isoenzymes alpha, beta, gamma, delta, and epsilon. Results suggest that alpha-thrombin and TRAP-6 activate at least partially the same intracellular signaling pathways in rat glioma C6 cells, which is evidence for involvement of "tethered ligand" receptor in thrombin induced signaling in glioma C6 cells.

• Authors: R Kaufmann, C Lindschau, A Höer, P Henklein, A Adomeit, H Haller, C Liebmann, E Oberdisse, G Nowak
• Journal: Journal of neuroscience research (J Neurosci Res) Vol. 46 Issue 6 Pg. 641-51 (Dec 15 1996) ISSN: 0360-4012 [Print] United States
• PMID: 8978498 (Publication Type: Journal Article)

Chemical References

• Fluorescent Dyes
• Inositol Phosphates
• Isoenzymes
• Peptide Fragments
• Receptors, Thrombin
• thrombin receptor peptide (42-47)
• Tritium
• Prkcd protein, rat
• Prkce protein, rat
• protein kinase C gamma
• PRKCA protein, human
• PRKCD protein, human
• PRKCE protein, human
• Protein Kinase C
• Protein Kinase C beta
• Protein Kinase C-alpha
• Protein Kinase C-delta
• Protein Kinase C-epsilon
• Thrombin
• Calcium

Topics

• Animals
• Calcium (metabolism)
• Enzyme Activation
• Fluorescent Dyes
• Glioma
• Humans
• Inositol Phosphates (pharmacokinetics)
• Isoenzymes (metabolism)
• Microscopy, Confocal
• Peptide Fragments (pharmacology)
• Protein Kinase C (metabolism)
• Protein Kinase C beta
• Protein Kinase C-alpha
• Protein Kinase C-delta
• Protein Kinase C-epsilon
• Rats
• Receptors, Thrombin (metabolism)
• Signal Transduction (drug effects)
• Thrombin (pharmacology)
• Time Factors
• Tritium
• Tumor Cells, Cultured (drug effects, enzymology)