Abstract |
Cold preservation of donor organs induces hypothermia-related tissue edema as a result of a reduced activity of the ATP-dependent sodium pump at low temperatures. Hypothermia-induced tissue edema occurs in kidney preservation and is a significant risk factor for delayed graft function (DGF) after transplantation. DGF remains a major problem in kidney transplantation and is significantly associated with preservation injury. The state of hydration of cold-stored organs can be assessed from a biopsy for determination of the wet/dry weight ratio. As a non-invasive method to determine tissue hydration MRI T1 and T2 relaxometry can be used. In this study we have compared changes in tissue hydration in UW-preserved porcine kidneys with increasing cold ischemia times ( CIT) using wet/dry weight ratio and MR ralaxometry. The results of the two techniques were correlated to evaluate the use of MR relaxometry. Wet/dry weight ratios of the renal cortex decreased with prolonged CIT (P < 0.01) whereas those of the medulla did not change significantly. T1 values of the cortex decreased with prolonged CIT (P < 0.01). T2 values of the cortex showed a non-significant decline with increased CIT. No significant changes in T1 and T2 were found in the medulla. The correlation between T1 and the wet/dry weight ratio of the cortex was significant (P = 0.05, linear correlation coefficient 0.8698). We conclude that MR relaxometry can be a valuable noninvasive technique to assess tissue hydration in cadaveric donor kidneys before transplantation.
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Authors | J A van der Hoeven, L J de Jong, R F Wolf, R L Kamman, R J Ploeg |
Journal | Transplant international : official journal of the European Society for Organ Transplantation
(Transpl Int)
Vol. 9 Suppl 1
Pg. S452-4
( 1996)
ISSN: 0934-0874 [Print] Switzerland |
PMID | 8959884
(Publication Type: Journal Article)
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Chemical References |
- Insulin
- Organ Preservation Solutions
- University of Wisconsin-lactobionate solution
- Allopurinol
- Glutathione
- Adenosine
- Raffinose
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Topics |
- Adenosine
- Allopurinol
- Animals
- Female
- Glutathione
- Insulin
- Kidney Cortex
(metabolism)
- Kidney Medulla
(metabolism)
- Kidney Transplantation
- Organ Preservation
- Organ Preservation Solutions
- Raffinose
- Swine
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