In vitro chemotherapy of steroid receptor positive human meningioma low-passage primary cultures with nitrosourea-methionine-steroid conjugates.

Abstract

The antiproliferative activity of two nitrosourea-methionine-steroid conjugates, N-[N'-(2-chloroethyl)-N'-nitrosocarbamoyl] (CNC)-L-methionine-testosterone-17-ester and CNC-L-methionine-dihydrotestosterone-17-ester, was investigated in two meningioma low-passage primary cultures that had been derived from human meningiomas and characterized with regard to proliferation pattern and progesterone receptor content. Treatment was given over 4 days. Cytotoxicity (concentrations investigated: 50, 100, and 200 microM) was compared with the effects of the equimolar mixtures of CNC-Met plus steroid. The steroid-linked nitrosoureas were superior to their unlinked components. CNC-Met itself displayed no or marginal activity, whereas the steroid component displayed significant activity.

• Authors: F J Taut, W J Zeller
• Journal: Clinical neuropharmacology (Clin Neuropharmacol) Vol. 19 Issue 6 Pg. 520-5 (Dec 1996) ISSN: 0362-5664 [Print] United States
• PMID: 8937791 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• N-(N'-(2-chloroethyl)--N'-nitrosocarbamoyl)methionine-dihydrotestosterone-17-ester
• N-(N'-(2-chloroethyl)-N'--nitrosocarbamoyl)methionine-testosterone-17-ester
• Nitrosourea Compounds
• Receptors, Steroid
• Testosterone

Topics

• Antineoplastic Agents (pharmacology)
• Drug Screening Assays, Antitumor
• Female
• Humans
• Meningeal Neoplasms (drug therapy, ultrastructure)
• Meningioma (drug therapy, ultrastructure)
• Middle Aged
• Nitrosourea Compounds (pharmacology)
• Receptors, Steroid (analysis)
• Testosterone (analogs & derivatives, pharmacology)
• Tumor Cells, Cultured