Simultaneous determination of bromhexine hydrochloride and methyl and propyl p-hydroxybenzoate and determination of dextromethorphan hydrobromide in cough-cold syrup by high-performance liquid chromatography.

Abstract

Liquid chromatographic methods were developed for the determination of bromhexine hydrochloride, methyl p-hydroxybenzoate and propyl p-hydroxybenzoate (method A) and dextromethorphan hydrobromide (method B) in cough-cold syrup formulations. Reversed-phase analytical columns (150 mm x 3.9 mm i.d.) were used with (A) C18 and (B) phenyl as stationary phases and mixtures of (A) acetonitrile and aqueous 15 mM triethylamine solution (43:57) and (B) methanol and aqueous 3% ammonium formate buffer solution (53:47) as mobile phases at a flow rate of 1.0 ml min-1. Both aqueous components were adjusted to pH 3.9. UV detection of analytes was at (A) 245 nm and (B) 278 nm. In both methods, the time required for an HPLC run giving good separations and recoveries was less than 8 min.

• Authors: J P Rauha, H Salomies, M Aalto
• Journal: Journal of pharmaceutical and biomedical analysis (J Pharm Biomed Anal) Vol. 15 Issue 2 Pg. 287-93 (Nov 1996) ISSN: 0731-7085 [Print] England
• PMID: 8933431 (Publication Type: Journal Article)

Chemical References

• Antitussive Agents
• Expectorants
• Parabens
• Dextromethorphan
• methylparaben
• Bromhexine
• propylparaben

Topics

• Antitussive Agents (analysis)
• Bromhexine (analysis)
• Chromatography, High Pressure Liquid (methods)
• Dextromethorphan (analysis)
• Expectorants (analysis)
• Parabens (analysis)