Ca2+ mobilization during cell death induction by sodium 5, 6-benzylidene-L-ascorbate.

Abstract

The cytotoxic activity of sodium 5,6-benzylidene-L-ascorbate (SBA) against human KG-1-C glioma and T98G glioblastoma cell lines was augmented by pretreatment of the cells with L-buthionine-[S, R]-sulfoximine (BSO), which reduced the intracellular glutathione concentrations. SBA produced shrunken cells and large DNA fragments, without the induction of nuclear and internucleosomal DNA fragmentation. The rapid elevation of intracellular free Ca2+ concentration observed after SBA treatment was further augmented by BSO pretreatment. A confocal experiment with Fluo-3 fluorescence revealed that SBA markedly elevated the free Ca2+ concentration in the nuclear region, but did not significantly affect that in the cytoplasmic region. The present study suggests that the nuclear accumulation of Ca2+ is an important initial step for cell death induction by SBA.

• Authors: H Takahashi, H Sakagami, H Ohata, M Iida, K Momose, M Yamamura, M Takeda
• Journal: Anticancer research (Anticancer Res) 1996 Sep-Oct Vol. 16 Issue 5A Pg. 2629-34 ISSN: 0250-7005 [Print] Greece
• PMID: 8917362 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Benzylidene Compounds
• DNA, Neoplasm
• Buthionine Sulfoximine
• Glutathione
• Ascorbic Acid
• Calcium
• zilascorb

Topics

• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects)
• Ascorbic Acid (analogs & derivatives, pharmacology)
• Benzylidene Compounds (pharmacology)
• Buthionine Sulfoximine (pharmacology)
• Calcium (metabolism)
• DNA, Neoplasm (drug effects)
• Glutathione (metabolism)
• HL-60 Cells (metabolism)
• Humans